The Biotechnology Separation Systems Market is projected to grow from USD 30544.49 million in 2023 to USD 43594.19 million by 2032, at a CAGR of 6.11%. Key players include Sartorius Stedim Biotech, Merck, Hitachi Koki, and others. The market is driven by technological innovation, surging demand, regulatory encouragement, environmental consciousness, and cost efficiency. Challenges include upfront costs, consistency issues, infrastructure needs, policy fluctuations, market competition, supply chain vulnerabilities, public acceptance, and knowledge gaps.