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Microscopic imaging and FRAP experiments reveal CTAB-BSA forms micron-sized liquid-like droplets with high mobility. Nucleotide presence affects droplet pH and mobility, with ATP showing stronger interaction with CTAB. Droplet stability and pH are influenced by nucleotide binding, with ATP significantly decreasing surface potential. Fluorescence spectroscopy using HPTS indicates nucleotides alter droplet pH, with ATP causing a sharp decrease. Confocal microscopy confirms ATP-induced pH changes and their impact on protein functionalization rates, particularly enhancing lysine-specific NBD functionalization. Enzymatic hydrolysis of ATP to AMP and P_i reverses these effects, demonstrating temporal control over protein modification in droplet states.

Study details the experimental model and subject details for five cases of isolated PM-ccRCC, including patient inclusion/exclusion criteria, sample transport, processing, and cell culture optimization. The study also covers exome sequencing, mutation and CN analysis, single-cell RNA sequencing, drug sensitivity and resistance testing, immunostainings, pathway analysis, and ethical approvals. Statistical analysis and reproducibility are discussed, with results compared between PM-ccRCC DSRT data and previously published ccRCC DSRT data.

HCT116 and LoVo cells were cultured in DMEM supplemented with 10% FBS and 1% antibiotics at 37°C with 5% CO₂. Transcription profiles and clinical info for colorectal adenocarcinoma (COAD) were downloaded from TCGA and GEO databases. SiRNAs and plasmids were transfected using Lipofectamine 2000. Single-cell transcriptome profiles were analyzed using Seurat, and gene set enrichment analysis (GSEA) was performed with GSEA software. WGCNA was used to determine gene co-expression modules. Western blotting, qRT-PCR, Transwell assay, ChIP-seq, and ChIP-qPCR were conducted to analyze protein and gene expression. FISH and immunofluorescence were used to detect LINC00887 RNA probe. Immunohistochemistry (IHC) was performed on CRC tissue chips. Animal experiments were approved by IACUC, and statistical analysis was done using GraphPad Prism software.