The Transcriptomic Study of Thai Patients With Atopic Dermatitis by Tape Strips
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- Immune biomarkers
研究概览
简要总结
This study will be use the tape strip technique to evaluate the skin biomarkers of atopic dermatitis among Thai patients to differentiate clinical phenotype.
详细描述
The patients will be enrolled in this study if they have been diagnosed with atopic dermatitis. All participants (AD patients and controls) will be evaluated their skin biomarkers by using tape stripping. The tape strips will be applied to the antecubital fossa to collect the epithelial samples. Then RNA was extracted from the tape strips for mRNA profiling to identify the immune and epidermal barrier genes.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 1 Year 至 60 Years（Child, Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Children (age 1-18 years old) with mild atopic dermatitis
- •Children (age 1-18 years old) with moderate to severe atopic dermatitis
- •Children (age 1-18 years old) with moderate to severe atopic dermatitis and food allergy
- •Adult (age 18-60 years old) with atopic dermatitis
- •Healthy individuals (1-60 years old)
- •Patients with asthma (1-60 years old)
排除标准
- •Active skin infections
- •Used systemic immunosuppressants within 4 weeks
- •Used topical steroids or immunomodulators within 1 week
- •Used moisturizers within 12 hours before evaluation
研究组 & 干预措施
Children with mild atopic dermatitis
- age 1-8 years old
- mild degree of atopic dermatitis
干预措施: Tape strips (Genetic)
Children with moderate-severe atopic dermatitis
- age 1-8 years old
- moderate or severe degree of atopic dermatitis
干预措施: Tape strips (Genetic)
Children with food allergy and moderate-severe atopic dermatitis
- age 1-8 years old
- moderate or severe degree of atopic dermatitis
- IgE mediated food allergy
干预措施: Tape strips (Genetic)
Adult with atopic dermatitis
- age 18-60 years old
- mild-severe atopic dermatitis
干预措施: Tape strips (Genetic)
Healthy
- age 1-60 years old
- no history of atopic diseases
干预措施: Tape strips (Genetic)
Healthy with Asthma
- age 1-8 years old
- doctor diagnosed asthma
- no history of chronic or chronic relapsing eczema
干预措施: Tape strips (Genetic)
结局指标
主要结局
Immune biomarkers
时间窗: 1 month
Evaluated by RNA sequencing: RNA was extracted for real-time polymerase chain reaction (RT-PCR) with the miRNAeasy Mini Kit (Qiagen, Hilden, Germany). Reverse transcription to complementary DNA (cDNA) from RNA was carried out using the High Capacity cDNA reverse transcription (Thermo fisher). TaqMan Low Density Array (TLDA) cards (Thermo fisher) were used for quantitative reverse transcription polymerase chain reaction (qRT-PCR). 500pg total RNA was used for PreAMP pool. Eukaryotic 18S recombinant RNA (rRNA) was used as an endogenous control. Expression values were normalized to Rplp0
Cellular AD biomarkers
时间窗: 1 month
Evaluated by RNA sequencing: RNA was extracted for real-time polymerase chain reaction (RT-PCR) with the miRNAeasy Mini Kit (Qiagen, Hilden, Germany). Reverse transcription to complementary DNA (cDNA) from RNA was carried out using the High Capacity cDNA reverse transcription (Thermo fisher). TaqMan Low Density Array (TLDA) cards (Thermo fisher) were used for quantitative reverse transcription polymerase chain reaction (qRT-PCR). 500pg total RNA was used for PreAMP pool. Eukaryotic 18S recombinant RNA (rRNA) was used as an endogenous control. Expression values were normalized to Rplp0
Barrier biomarkers
时间窗: 1 month
chain reaction (RT-PCR) with the miRNAeasy Mini Kit (Qiagen, Hilden, Germany). Reverse transcription to complementary DNA (cDNA) from RNA was carried out using the High Capacity cDNA reverse transcription (Thermo fisher). TaqMan Low Density Array (TLDA) cards (Thermo fisher) were used for quantitative reverse transcription polymerase chain reaction (qRT-PCR). 500pg total RNA was used for PreAMP pool. Eukaryotic 18S recombinant RNA (rRNA) was used as an endogenous control. Expression values were normalized to Rplp0
次要结局
未报告次要终点
研究者
Tassalapa Daengsuwan
Assistant Professor
Queen Sirikit National Institute of Child Health