Cryogenic Preservation of Spermatozoa : Comparative Study Between Both Dry and Liquid Phase Nitrogen Storages
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 52
- 试验地点
- 1
- 主要终点
- Sperm DNA integrity
研究概览
简要总结
The reference technique for the conservation of gametes is storage in liquid nitrogen but new vats of nitrogen vapor (storage over liquid nitrogen) or in dry phase (storage in an insulated compartment of liquid nitrogen in a tank Liquid nitrogen) also allow the storage of flakes. The purpose of this work is to evaluate the dry-phase cryopreservation technique of liquid nitrogen compared with liquid-phase storage, depending on the duration of cryopreservation.
详细描述
Objective
To evaluate the effects of cryopreserved sperm in dry and liquid phase nitrogen at 3 and 6 month on sperm numeration, motility, vitality, morphology, acrosomal integrity and DNA fragmentation.
Design: Experimental study, investigator was blinded to the type of Cryopreservation.
Patient(s): Semen samples were collected from patients who came in laboratory for semen analysis
Intervention: Samples were frozen with a programmable freezing unit. Each semen sample was divided into two aliquots. One aliquot was plunged into liquid nitrogen and the other was stored in dry-phase nitrogen for 3 or 6 month. Thawing was performed at room temperature.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Men undergoing routine semen analysis for infertility for reproductive medicine of university hospital in Clermont-Ferrand
排除标准
- 未提供
结局指标
主要结局
Sperm DNA integrity
时间窗: at 6 months
the spermatic DNA integrity can be quantified by TUNEL method. The result will be expressed as a percentage of fragmented DNA
次要结局
- Acrosomal integrity(at 3 and 6 months)
- Sperm parameter post cryopreservation(at 3 and 6 months)
- Lab parameter post cryopreservation(at 3 and 6 months)
- Spermatic DNA Compaction(at 3 and 6 months)