Assessment of Diagnostic Accuracy of Indocyanine Green Fluorescence Imaging in Children and Young People With Testicular Pain Requiring Surgical Exploration Is ICG Imaging Safe and Accurate to Predict Testicular Torsion?

Children being taken to theatre for testicular pain for suspected torsion over a 1-year period will be registered prospectively. A clerking proforma will be utilised for all children with testicular pain with details including duration of pain, presentation pathway, address, dob, ethnicity, tanner stage, comorbidities and the standard questions in a clerking for children with testicular pain.

Children proceeding to theatre will be consented for injection of ICG within their consent for surgery by appropriately GCP trained and delegated study team members. . Age-appropriate information leaflets will be provided, and consent and assent taken as appropriate from potential participants and their guardians (if required). Children having consented will have allocation of a participant ID. They will then proceed for injection of indocyanine green after safe establishment of anaesthetic and with the surgical team present. After 5 minutes an image of the CYP scrotum will be taken prior to prepping. The images are taken using a specialist near infra-red (NIR) camera. This is a new imaging system for SCNFT, but technology used at Sheffield Teaching Hospitals and in other children's hospitals in the country. This NIR camera head requires a new stacker for theatres but is fully compatible with our systems. These images will be taken under the category ICG scrotum on the theatre tablets. These will be stored on a tablet and printed from the theatre stack and exported to the server as standard practice for all images in the surgical department. Participant ID will be attached to the images and the images will be stored in the electronic notes. Process in theatre in described in Appendix 3.

The findings at surgery will be recorded and describe torsion, degree of torsion, colour at surgery, bleed time. A second image will be taken in cases of torsion. Appendix 2, form 2 describes data collection taken in theatre. Once detorted a second image will be taken and processed similarly. Fixation will proceed as standard practice.

There will be no change in the patient pathway for acute scrotum. Any adverse events will be monitored via immediate reporting of side effects to PI Caroline MacDonald, see Appendix for theatre protocol.

All children with torsion will have a follow up phone call at 12 months to check testicular status and any adverse events. Those without torsion do not need long term follow up, as we do not expect late atrophy.