NCT04470505已完成不适用
Optimizing Platelet Isolation for Measures of Human Platelet Mitochondrial Respiration (The Cell Study)
适应症
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 16
- 试验地点
- 1
- 主要终点
- Platelet activation from isolated platelets
研究概览
简要总结
The research study is about blood cells (platelets) and their power houses (mitochondria). The investigators want to learn about their behavior in order to ensure that our laboratory procedures are generating optimal results.
详细描述
The objectives of this study are to assess platelet activation following standard laboratory procedures used in the process of measuring platelet mitochondrial function, and to determine the optimal amount of digitonin needed to permeabilize platelet cell membrane.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 25 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age 25-35
- •All races
- •All ethnicity
- •18.5 - 30 BMI
排除标准
- •Aspirin intake
- •Recreational drug use
- •Alcohol intake more than 3 times per week
- •Self-reported possibility of current pregnancy
- •Known diagnosis of:
- •Pre-diabetes
- •High cholesterol
- •High triglycerides
- •Hypertension
- •Autism spectrum disorder
- •Attention deficit hyperactivity disorder (ADHD)
- •Attention deficit disorder (ADD)
- •Severe asthma
- •Refusal to stop taking nutritional supplements
- •Any other pre-existing medical condition that requires daily medication as determined by the investigators to affect outcome of interest
结局指标
主要结局
Platelet activation from isolated platelets
时间窗: day 1
Whole blood will be collected following an overnight fast, and platelets isolated using standard procedures. Platelet activation markers (e.g., CD62P ) will be measured using flow cytometric analysis.
次要结局
- Platelet activation measured in whole blood(day 1)
- Platelet reactivity measured in whole blood(day 1)
研究者
研究点 (1)
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