Metabolic Adaptation to Plant-based Diets in Asian Populations
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 57
- 试验地点
- 1
- 主要终点
- Genotype at SNP rs9366637
研究概览
简要总结
The two specific aims of this study are: 1) to assess the impact of a genetic variant on iron status; 2) to assess the impact of a genetic variant on non-heme absorption among Asian women. The investigators hypothesize that the genetic variant could enhance iron status and iron absorption in Asian women.
详细描述
To determine the HFE genotype of female Asian study volunteers, we collected venous blood samples and extracted DNA from these blood samples. HFE genotype was determined as CC, CT, and TT and used to assess possible differences in iron status as a function of genotype. To measure the impact of genotype on non-heme Fe absorption, a sub-group of women with the CC (n=10) or TT (n=11) genotype were invited to return for an Fe absorption study. Each volunteer consumed stable 57Fe (as ferrous sulfate). Two weeks after ingesting this tracer, a blood sample was collected from each woman and the amount of non-heme (57Fe) iron incorporated into red blood cells was measured with magnetic sector thermal ionization mass spectrometry. Possible associations between iron status and iron absorption as a function of genotypes were explored. Data from this study will provide information that aims to improve human health by better understanding the iron requirements of individuals with different genetic background.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •non-pregnant and between the ages of 18~35 y
- •East Asian descent with both maternal and paternal grandparents from East Asia, including China, Japan, South Korea, Vietnam, Thailand, Malaysia and Singapore
排除标准
- •taking or planing to take any vitamin or mineral supplements during the study period
- •pre-existing medical problems that might impact inflammation or Fe status, including malabsorption, blood disorders, ulcers, joint diseases and asthma
- •taking any prescribed medications known to affect iron homeostasis
结局指标
主要结局
Genotype at SNP rs9366637
时间窗: 2 weeks after the participant's visit to the laboratory
At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining genotype.
Serum transferrin receptor
时间窗: Up to 6 months after the participant's visit to the laboratory
At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of serum transferrin receptor.
Hemoglobin
时间窗: Up to 2 days after the participant's visit to the laboratory
At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of hemoglobin.
Serum Ferritin
时间窗: Up to 6 months after the participant's visit to the laboratory
At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of serum ferritin.
Non-heme Iron absorption in women with 2 HFE genotypes
时间窗: one month for screening with genotype then 2 weeks for iron absorption study
Women donated a blood sample for genotyping. Women with particular HFE genotypes were invited to return for an absorption study. Two weeks after women consume the iron tracer, a blood sample was obtained to measure the amount of iron absorbed.
次要结局
- C-reactive protein(Up to 6 months after the participant's visit to the laboratory)
- Vitamin B-12(Up to 6 months after the participant's visit to the laboratory)
- Folate(Up to 6 months after the participant's visit to the laboratory)