A Histo-immunological Comparative Study of Bone Cutting by Conventional Surgery and Piezosurgery on the Secretion of Heat Shock Protein 70 (HSP70) and on the Bone Cells
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 15
- 试验地点
- 2
- 主要终点
- Levels of Heat Shock Protein 70
研究概览
简要总结
Evaluation of the effect of conventional and piezo-surgery on the secretion of Heat Shock Protein (HSP70) and on the vial bone cells at the peripheral bone cut sides after the removal of impacted mandibular third molars on both sides of the jaw.
详细描述
The study is about measuring the amount of HSP70 using Elisa kit for detecting HSP70 and mason trichrome for histological findings in which the study samples are patients who have bilateral impacted mandibular third molar to be extracted with bone removal. First we collect bone sample for mason trichrome with either method then we collect the second samples after removing most of the bones cover the impacted third molar for checking HSP70 levels. Then the bone samples are treated using a special protocol to eliminate the protein from bone samples for the Elisa analysis. The other bone samples are going to be studied under the microscope.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 16 Years 至 35 Years（Child, Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •bilateral identical impacted third molar that need at least 3 mm of bone removal
排除标准
- •Pregnancy
- •Infectious diseases
- •Infections around third molars (i.e. pericoronitis).
- •Alcoholic patients
研究组 & 干预措施
Conventional Surgery
No intervention in this arm. The patients are going to undergo normal surgical intervention. Conventional surgery will be performed to extract the impacted third molars.
Piezosurgery
Patients here will undergo a piezosurgery to extract their third molars on both sides.
干预措施: Piezosurgery (Device)
结局指标
主要结局
Levels of Heat Shock Protein 70
时间窗: Five minutes before third molar removal with the last removed layer of bone
This will be measured using ELISA methodology
次要结局
- Percentage of viable bone cells(This variable will be measured from the specimens taken within the first 15 minutes of bone cutting to remove third molars)