Stem Cell-based Approaches to Neuronal Characteristics and Endophenotype of Schizophrenia in Genetic High Risk Subjects
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 1
- 主要终点
- Structural and functional characteristics of neurons differentiated from adipose tissue derived stem cells in subjects with genetic high risk for schizophrenia and healthy controls
研究概览
简要总结
This study aims at finding endophenotypes of schizophrenia at neuronal level by obtaining stem cells which is derived from adipose cells of subjects with heavy genetic loading for schizophrenia then differentiating them into neuronal cells.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 20 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •[Subjects at genetic high risk for schizophrenia]
- •Healthy without any Axis I mental disorder
- •Is a monozygotic twin of a patient with schizophrenia OR Has at least two family members of schizophrenia in the pedigree, including at least one 1st-degree family member
- •[Healthy Control]
- •Healthy without any Axis I mental disorder
- •No family members of schizophrenia in the pedigree to the 3rd degree
排除标准
- •Significant neurological or medical illness
- •Psychotic symptoms
- •Substance abuse
- •Suicidal risk
- •Blindness or hearing loss
- •Taking aspirin, warfarin or hormonal agents
- •Pregnancy or lactation
- •Susceptibility for keloid formation
- •Allergy to lidocaine
- •History of significant head trauma or loss of consciousness
- •Mental retardation
结局指标
主要结局
Structural and functional characteristics of neurons differentiated from adipose tissue derived stem cells in subjects with genetic high risk for schizophrenia and healthy controls
时间窗: three years
in vitro measurement of the expression of the neuronal markers for differentiated post-mitotic neuron, GABAergic/Glutamatergic neuron. The neuronal connectivity, neurites from soma and synaptic protein levels will be assessed. In addition, RNA and proteins expression (e.g. Glutamate/GABA receptors) , physiological function, and in vitro response to antipsychotics will be evaluated.
次要结局
- PANSS(Positive and Negative Syndrome Scale)(Baseline)
- PET imaging data(Baseline)
- Neurocognitive function test battery (composite)(Baseline)
- ERP(event-related potential) profile(Baseline)
- Structural/resting functional MRI data(Baseline)
- Proton MR spectroscopy(Baseline)
- CAARMS(Comprehensive assessment of at risk mental states)(Baseline)