The Effects of Pranayamic Breathing Techniques and Tongue Exercises on Oral Microbiota in Children Aged 7-12 Years
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 80
- 试验地点
- 1
- 主要终点
- Change in oral microbiota alpha diversity as measured by the Shannon diversity index from 16S rRNA gene sequencing of saliva samples
研究概览
简要总结
This study aims to evaluate the effects of pranayamic breathing techniques and tongue exercises on oral microbiota in children aged 7-12 years. Children will be grouped according to their breathing patterns (mouth or nasal breathing), and a structured breathing and tongue exercise program will be applied. Saliva samples will be collected before and after the intervention to analyze changes in oral microbiota. The study will include approximately 80 participants and is expected to last for 6 months. There are no anticipated risks for participants. The findings of this study may contribute to a better understanding of the relationship between breathing patterns, oral exercises, and oral health in children.
详细描述
This study is designed to investigate the effects of pranayamic breathing techniques, tongue exercises, and tongue cleaning on oral microbiota in children aged 7-12 years. Participants will be classified based on their breathing patterns as mouth breathers or nasal breathers. Each group will undergo a structured intervention program that includes specific breathing techniques, tongue exercises, and, in selected subgroups, tongue cleaning over a defined period.
Saliva samples will be collected from all participants before and after the intervention. These samples will be analyzed using microbiological methods to evaluate changes in oral microbiota composition. The study aims to compare the microbiological outcomes between different breathing patterns and to assess the potential benefits of the applied interventions.
Informed consent will be obtained from all participants and their legal guardians prior to participation. All collected samples and data will be anonymized to ensure participant confidentiality.
This research is expected to provide insights into the relationship between breathing habits, oral functions, tongue hygiene practices, and oral microbial balance in children. The findings may contribute to preventive and therapeutic approaches in pediatric dentistry, particularly in managing the effects of mouth breathing on oral health.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Outcomes Assessor)
盲法说明
The outcomes assessor responsible for microbiota analysis will be blinded to group allocation. Saliva samples will be coded and anonymized to prevent identification of participants' group assignments.
入排标准
- 年龄范围
- 7 Years 至 12 Years（Child）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Children aged 7-12 years with either mouth breathing or nasal breathing patterns
- •Willingness of both the child and their legal guardian to participate in the study
- •Children who are cooperative and able to comply with the exercise protocol
排除标准
- •Refusal of the child or legal guardian to participate in the study
- •Presence of active dental caries or periodontal disease
- •Presence of any systemic disease or regular medication use
- •Use of orthodontic appliances
- •Use of antibiotics or probiotics within the last 4 weeks
研究组 & 干预措施
Mouth Breathing Group
Participants with mouth breathing habits will be included in this group. A structured program consisting of pranayamic breathing techniques, tongue exercises, and, in selected subgroups, tongue cleaning will be applied. Saliva samples will be collected before and after the intervention to evaluate changes in oral microbiota.
干预措施: Pranayamic Breathing and Tongue Exercises (Behavioral)
Nasal Breathing Group
Participants with nasal breathing habits will be included in this group. A structured program consisting of pranayamic breathing techniques, tongue exercises, and, in selected subgroups, tongue cleaning will be applied. Saliva samples will be collected before and after the intervention to evaluate changes in oral microbiota.
干预措施: Pranayamic Breathing and Tongue Exercises (Behavioral)
结局指标
主要结局
Change in oral microbiota alpha diversity as measured by the Shannon diversity index from 16S rRNA gene sequencing of saliva samples
时间窗: 8 weeks
This outcome evaluates the effect of the intervention (pranayamic breathing exercises, tongue exercises, and tongue-cleaning practice) on the diversity of the oral microbiota. Alpha diversity will be assessed from saliva samples using 16S rRNA gene sequencing and quantified using the Shannon diversity index. The mean Shannon index at baseline will be compared with the value after the 8-week intervention, and the change from baseline will be reported.
次要结局
- Salivary Nitric Oxide Levels(8 weeks)
研究者
Gulcin Ozsen
Principal Investigator
Istanbul Health and Technology University