The Influence of Motile Sperm Organelle Morphology Examination on ICSI Outcomes
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 72
- 试验地点
- 2
- 主要终点
- Pregnancy rates
研究概览
简要总结
A new technique based on the motile sperm organelle morphology examination (MSOME) at a magnification of at least ×6000 has been developed. The MSOME assesses the nucleus, for both shape and chromatin content, which is the most important sperm organelle in influencing intracytoplasmic sperm injection (ICSI) outcomes.
详细描述
Sperm morphology has been described as one of the major determinants of male in vitro fertility. However, the standard morphology evaluation on random stained cells from the ejaculate is of limited value during ICSI. Current research is directed towards the dysmorphisms of the sperm head.A new technique based on the motile sperm organelle morphology examination (MSOME) at a magnification of at least ×6000 has been developed. The MSOME assesses the nucleus, for both shape and chromatin content, which is the most important sperm organelle in influencing ICSI outcomes. The goals for this study are to identify whether there is a connection between morphologic sperm normalcy under high magnification and (i) semen analysis according to the new World Health Organization (WHO) reference values and (ii) ICSI outcomes.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Diagnostic
- 盲法
- Double (Participant, Outcomes Assessor)
入排标准
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Couples undergoing ICSI as result of male factor or tubal factor or unexplained infertility
- •Women with regular menstrual cycles of 25-35 days
- •Women with normal basal follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels
- •Women with body mass index (BMI) less than 30 kg/m2
- •Women with both ovaries and intact uterus
排除标准
- •Women with polycystic ovaries syndrome
- •Women with endometriosis
- •Women with gynaecological/medical disorders
- •Couples with any sexually transmitted disease
- •Women who had received any hormone therapy for less than 60 days preceding the study
研究组 & 干预措施
MSOME
The samples will be evaluated under high magnification
干预措施: MSOME (Other)
结局指标
主要结局
Pregnancy rates
时间窗: 1 month
Number of clinical pregnancy rate divided by the number of couples in which embryo transfer was performed.
次要结局
- Implantation rates(1 month)