Effect of Age on Recipient Site Wound Healing in Free Gingival Graft Operation
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- Shrinkage percentage (%)
研究概览
简要总结
This study aimed to assess the effects of age on wound healing on free gingival graft (FGG) surgery in the treatment of attached keratinized gingival deficiency by determining clinical and biochemical outcomes.
详细描述
Aim of this study was to evaluate the effect of age on clinical and biochemical healing outcomes on FGG surgery in the treatment of attached keratinized gingival deficiency (≤2 mm) at mandibular anterior region. A total of 30 systemically healthy patients, divided into two groups and planned for FGG operations, were included in this study. These groups are; Group l : 15 patients in the age range of 20-35, group 2: 15 patients in the age range of 50-65. Gingival crevicular fluid and wound fluid samples were taken from these patients to analyze VEGF, EGF and TGF-B1 levels by ELISA. Wound fluid samples were taken baseline, 4,7, and 14 days after surgery.
Clinical parameters included measurement of probing depth (PD), clinical attachment level (CAL), bleeding of probing (BOP), plaque index (PI), gingival index (GI) at 6 sites per tooth except the third molars were measured at baseline and l, 3 and 6 months after surgery. Keratinized gingiva width, keratinized gingiva thickness of the operation sites were measured baseline and 1,3 and 6 months after surgery. Vertical dimension of the graft, horizontal dimension of the graft, graft thickness were measured at baseline and 1,3,6 months after surgery. Graft area measurements were calculated on standard photographs by a Java-based analysis program (ImageJ, National Institutes of Health, Bethesda, MD) to determine the shrinkage rate of the grafts. The graft shrinkage ratio was calculated as the difference between baseline to 1-month, baseline to 3-months, baseline to 6-month graft site value. Data were analyzed using appropriate statistical tests.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Outcomes Assessor)
入排标准
- 年龄范围
- 20 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •having ≤2mm of keratinized gingiva in at least 1 mandibular anterior tooth
- •periodontally healthy with full-mouth bleeding and plaque score ≤ 10%
- •no use of any drugs that will affect periodontal tissue health and healing process
- •absence of any systemic condition or illness
排除标准
- •previous periodontal surgery
- •a pathology in the apical region of the tooth
- •pregnancy, lactation
研究组 & 干预措施
Young group: Aged 20-35 years
They received free gingival operation. Graft was harvested from palatal region and placed to the recipient site. Wound fluid samples were collected from 4 regions of the wound at baseline, 4-,7-,14-days. Clinical measurements were done at baseline, 1-,3-,6-months postoperatively.
干预措施: Free gingival graft operation (Procedure)
Adult group: Aged 50-65 years
They received free gingival operation. Graft was harvested from palatal region and placed to the recipient site. Wound fluid samples were collected from 4 regions of the wound at baseline, 4-,7-,14-days. Clinical measurements were done at baseline, 1-,3-,6-months postoperatively.
干预措施: Free gingival graft operation (Procedure)
结局指标
主要结局
Shrinkage percentage (%)
时间窗: from baseline to 1-,3-,6- months
Shrinkage percentage of the graft area due to the changes in graft dimensions
次要结局
- VEGF levels (pg/ml)(from baseline to 1-,3-,6- months)
- EGF levels (pg/ml)(baseline to 1-,3-,6- months)
- TGFβ-1 levels (pg/ml)(from baseline to 1-,3-,6- months)