The Influence of Sampling Site When Assessing Glucose Tolerance or Insulin Sensitivity With Oral Glucose Ingestion
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- Postprandial plasma glucose concentrations (area under the concentration-time curve)
研究概览
简要总结
For decades, it has been known that post-meal blood glucose concentrations were associated with the risk of T2D, which was reflected in early diagnostic guidelines. The oral glucose tolerance test (OGTT) has been used since at least 1923 and has remained the most common test for assessing glucose tolerance. Arterial blood (or arterialised blood using heated hand technique) is most appropriate for determining glucose tolerance and insulin sensitivity since this best represents the concentrations of metabolites and hormones that peripheral tissues are exposed to. It is essential to investigate whether venous blood (sometimes used during an OGTT) is representative of arterialised blood during an OGTT, and under different metabolic conditions.
The investigators want to understand whether OGTT-derived insulin sensitivity indices differ from venous and arterialised blood; and 2) investigate whether metabolic status (i.e. rest vs lower-limb exercise) influences the difference between forearm venous and arterialised concentrations of glucose and insulin during an OGTT.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 49 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Aged 18-49 years
- •Able and willing to safely comply with all study procedures
- •Able to provide written informed consent for participation
排除标准
- •Diagnosis of any bleeding disorder or taking medication which impacts blood coagulation
- •The presence of any contraindications to maximal exercise testing, as determined using a physical activity readiness questionnaire (PAR-Q).
- •Any diagnosis of metabolic disease (i.e. cardiovascular disease or type 2 diabetes).
研究组 & 干预措施
Rest
Remain rested prior to the oral glucose tolerance test
干预措施: Rest (Behavioral)
Rest
Remain rested prior to the oral glucose tolerance test
干预措施: Dorsal Hand Vein Cannulation (heated hand technique) (Other)
Rest
Remain rested prior to the oral glucose tolerance test
干预措施: Venous Cannulation (Other)
Exercise
Complete exercise prior to the oral glucose tolerance test
干预措施: Dorsal Hand Vein Cannulation (heated hand technique) (Other)
Exercise
Complete exercise prior to the oral glucose tolerance test
干预措施: Venous Cannulation (Other)
Exercise
Complete exercise prior to the oral glucose tolerance test
干预措施: Exercise (Behavioral)
结局指标
主要结局
Postprandial plasma glucose concentrations (area under the concentration-time curve)
时间窗: 120 mins
OGTT-derived insulin sensitivity
时间窗: 120 mins
次要结局
- Fasting plasma glucose concentrations(5 mins)
- Substrate utilization for energy provision(180 mins)
- Fasting plasma insulin concentrations(5 mins)
- Fasting plasma lactate concentrations(5 mins)
- Fasting plasma triglyceride concentrations(5 mins)
- Postprandial plasma lactate concentrations (area under the concentration-time curve)(120 mins)
- Postprandial plasma insulin concentrations (area under the concentration-time curve)(120 mins)
- Postprandial plasma triglyceride concentrations (area under the concentration-time curve)(120 mins)
研究者
Javier Gonzalez
Lecturer
University of Bath