跳至主要内容
MedPath Trial logoMedPath TrialMedPath Trial Home
临床试验/NCT03033953
NCT03033953已完成不适用

Effects of Native Whey and Milk Supplementation on Changes in Muscle Mass and Strength After 12 Weeks of Strength Training in Young and Elderly

Norwegian School of Sport Sciences2 个研究点 分布在 1 个国家目标入组 66 人开始时间: 2014年8月1日最近更新:
Share to TwitterShare to FacebookShare to LinkedInShare to Reddit
适应症

试验速览

阶段
不适用
状态
已完成
发起方
入组人数
66
试验地点
2
主要终点
Lean mass
研究概览研究设计入排标准结局指标研究者研究点记录与标识符相似试验

研究概览

简要总结

The aim of this study is to investigate the long term adaptations to 11 (elderly) or 12 (young) weeks of strength training when supplemented with native whey or milk. The investigators hypothesize that native whey will give greater increases in muscle hypertrophy and strength than milk.

详细描述

Increasing or maintaining muscle mass is of great importance for populations ranging from athletes to patients and elderly. Resistance exercise and protein ingestion are two of the most potent stimulators of muscle protein synthesis. Both the physical characteristic of proteins (e.g. different digestion rates of whey and casein) and the amino acid composition, affects the potential of a certain protein to stimulate muscle protein synthesis. Given its superior ability to rapidly increase blood leucine concentrations to high levels, whey is often considered the most potent protein source to stimulate muscle protein synthesis. Native whey protein is produced by filtration of unprocessed milk. Consequently, native whey has different characteristics than milk. Of special interest is the higher amounts of the highly anabolic amino acid leucine in native whey.

The higher levels of leucine can be of great interest for elderly individuals as some studies in elderly has shown an anabolic resistance to the effects of protein feeding and strength training. By increasing levels of leucine one might overcome this anabolic resistance in the elderly.

The aim of this double-blinded, randomized, partial cross-over study is to compare the changes in muscle hypertrophy and strength after a 11 (elderly) or 12 (young) week training intervention with daily supplementation of either 2x20g of native whey or milk proteins in young and elderly individuals. In order to explain potential differences between supplements an acute study investigating acute responses in blood amino acid concentrations and intracellular signalling is planned, in a subgroup of participants, before and after the training intervention.

The investigators hypothesize that native whey will induce greater muscle hypertrophy and strength gains than milk.

研究设计

研究类型
Interventional
分配方式
Randomized
干预模型
Parallel
主要目的
Basic Science
盲法
Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)

入排标准

年龄范围
20 Years 至 —（Adult, Older Adult）
性别
All
接受健康志愿者
是

入选标准

  • •Healthy in the sense that they can conduct training and testing
  • •Able to understand Norwegian language written and oral
  • •Between 20 and 45, or above 70 years of age

排除标准

  • •Diseases or injuries contraindicating participation
  • •Use of dietary supplements (e.g. proteins, vitamins and creatine)
  • •Lactose intolerance
  • •Allergy to milk
  • •Allergy towards local anesthetics (xylocain)

结局指标

主要结局

Lean mass

时间窗: After 11-12 weeks of strength training

Measured by whole body dual x-ray absorptiometry (DXA)

次要结局

  • Thigh muscle cross sectional area(After 11-12 weeks of strength training)
  • 1 repetition maximum in bench press(After 11-12 weeks of strength training)
  • Phosphorylation of phosphorylated to total eukaryotic translation initiation factor 4E-binding protein 1 (4EBP-1) change from baseline(30 min before and 2 hours after exercise and protein supplement intake, after 11-12 weeks of strength training)
  • Plasma amino acid concentration(60 min before and 45, 60, 75 and 120 min after exercise and protein supplement intake, after 11-12 weeks of strength training)
  • Muscle force generating capacity change from baseline(15 min before, 10 min, 2.5 hours and 24 hours after exercise and protein supplement intake, after 11-12 weeks of strength training)
  • Serum insulin(60 min before and 45, 60, 75 and 120 min after exercise and protein supplement intake, after 11-12 weeks of strength training)
  • Serum creatine kinase(60 min before and 45, 60, 75 and 120 min after exercise and protein supplement intake, after 11-12 weeks of strength training)
  • Tumor necrosis factor alfa messenger ribonucleic acid (mRNA)(30 min before exercise and protein intake, before training intervention)
  • Thickness of the lateral vasti(After 11-12 weeks of strength training)
  • Serum glucose(60 min before and 45, 60, 75 and 120 min after exercise and protein supplement intake, after 11-12 weeks of strength training)
  • Phosphorylation of phosphorylated to total eukaryotic elongation factor 2 (eEF-2) change from baseline(30 min before and 2 hours after exercise and protein supplement intake, after 11-12 weeks of strength training)
  • Serum urea(60 min before and 45, 60, 75 and 120 min after exercise and protein supplement intake, after 11-12 weeks of strength training)
  • Interleukin 8 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Nuclear Receptor Subfamily 4 Group A Member 2 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Arm cross sectional area(After 11-12 weeks of strength training)
  • Interleukin 1b mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Ratio of phosphorylated to total ribosomal protein S6 kinase beta-1(P70S6K) change from baseline(30 min before and 2 hours after exercise and protein supplement intake, after 11-12 weeks of strength training)
  • C-C Motif Chemokine Ligand 2 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Pectoralis muscle cross sectional area(After 11-12 weeks of strength training)
  • 1 repetition maximum in leg press(After 11-12 weeks of strength training)
  • Cholesterol 25-Hydroxylase mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • C-C Motif Chemokine Ligand 8 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • C-C Motif Chemokine Ligand 3 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Nuclear Receptor Subfamily 4 Group A Member 3 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Nuclear Receptor Subfamily 1 Group H Member 3 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Myosin Heavy Chain 2 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Striated Muscle Activator Of Rho-Dependent Signaling mRNA Myosin Heavy Chain 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Fasting cholesterol (only in elderly)(After 11-12 weeks of strength training)
  • Tumor necrosis factor alfa mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Interleukin 4 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • C-X-C Motif Chemokine Ligand mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Interleukin 17D mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • C-C Motif Chemokine Ligand 5 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Myostatin mRNA Myosin Heavy Chain 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • TATA-Box Binding Protein mRNA Myosin Heavy Chain 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Beta-2-Microglobulin mRNA Myosin Heavy Chain 1 mRNA(30 min after exercise and protein intake, after 11-12 weeks of strength training)
  • Fasting LDL (only in elderly)(After 11-12 weeks of strength training)
  • Interleukin 6 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Interleukin 1 Receptor Antagonist mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Interleukin 10 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Peroxisome Proliferator-Activated Receptor Gamma, Coactivator 1 Alpha mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • C-X-C Motif Chemokine Ligand 16 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • ATP Binding Cassette Subfamily A Member 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Solute Carrier Family 3 Member 2 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Muscle-Specific RING Finger Protein 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Forkhead Box O3 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Mast Cell Growth Factor mRNA Myosin Heavy Chain 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • JunB Proto-Oncogene, AP-1 Transcription Factor Subunit mRNA Myosin Heavy Chain 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Colony Stimulating Factor 3 mRNA Myosin Heavy Chain 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Fasting triglycerides (only in elderly)(After 11-12 weeks of strength training)
  • Fasting HDL (only in elderly)(After 11-12 weeks of strength training)
  • Matrix Metallopeptidase 9 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Solute Carrier Family 7 Member 5 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Solute Carrier Family 38 Member 2 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Forkhead Box Protein O1A mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Myosin Heavy Chain 1 mRNA Myosin Heavy Chain 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Hepatocyte Growth Factor mRNA Myosin Heavy Chain 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Actin, Alpha 1, Skeletal Muscle mRNA Myosin Heavy Chain 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Filamin B mRNA Myosin Heavy Chain 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Uncoupling Protein 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Leukocyte Differentiation Antigen CD36 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Toll Like Receptor 2 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Solute Carrier Family 36 Member 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Atrogin-1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Myosin Heavy Chain 7 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Myosin Heavy Chain 1mRNA Myosin Heavy Chain 1 mRNA(30 min before exercise and protein intake, after 11-12 weeks of strength training)
  • Insulin Like Growth Factor 1 mRNA Myosin Heavy Chain 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)
  • Prostaglandin-Endoperoxide Synthase 2 mRNA Myosin Heavy Chain 1 mRNA(2 hours after exercise and protein intake, after 11-12 weeks of strength training)

研究者

发起方
Norwegian School of Sport Sciences
申办方类型
Other
责任方
Principal Investigator
主要研究者

Truls Raastad

Professor

Norwegian School of Sport Sciences

研究点 (2)

Loading locations...

相似试验

招募中
不适用
Effect of 12-week Milk Fat Globule Membrane Supplementation on the Human Muscle Function of Healthy, Recreationally Active Adults
NCT06573749Loughborough University96
已完成
不适用
Effects of Whey Protein in Type 2 DiabeticsType 2 Diabetes
NCT01944449Tel Aviv University60
已完成
不适用
Effects of 12-week dairy-derived nutritional supplementation combined with physical activity on health and physical function in healthy middle aged women after a 2-week period of reduced activityPhysical healthBone healthMental healthPhysical functionMusculoskeletal - Normal musculoskeletal and cartilage development and functionMetabolic and Endocrine - Normal metabolism and endocrine development and functionDiet and Nutrition - Other diet and nutrition disorders
ACTRN12616001714471Anthony Blazevich100
进行中（未招募）
不适用
Daily Protein Pacing Effects on HbA1c in Type 2 DiabeticsType 2 DiabetesHbA1cProtein Intake
NCT05355090Skidmore College12
尚未招募
不适用
Effects of Milk Powder Intervention on Body Composition and Physical Performance in Middle-aged and Older AdultsHealthy
NCT06429410Huilian Zhu200