Differential Diagnostics of Etiology of Acute Infections Based on the Analysis of Expression of Activation Markers on the Surface of Peripheral Blood Monocytes and Neutrophils.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 169
- 主要终点
- Sensitivity of the diagnosis of bacterial vs viral acute infection
研究概览
简要总结
Method for diagnostics of the origin of infections (bacterial vs viral) based on the identification of activation markers of blood neutrophils and monocytes will be developed.
详细描述
During the study, method for diagnostics of the origin of infections (bacterial vs viral) based on the identification of activation markers of blood neutrophils and monocytes will be developed.This method will allow to make a diagnosis within an hour after blood sampling.
The expression of the following markers of activation will be studied using flow cytometry: CD11b, CD11c, CD13, CD16, CD32, CD35, CD38, CD62L, CD64, CD66b, CD88, CD284, HLA-ABC and HLA-DR.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •neuroinfections or acute intestinal infections;
- •the possibility of blood sampling within a period of up to 24 hours from the moment of admission to hospital;
- •less than 72 hours passed after the onset of the disease and until blood was collected;
排除标准
- •refuse of patient to participate in the trial;
- •more than 24 hours passed after the patient entered the hospital;
- •the disease began more than 72 hours ago;
- •concomitant severe acute and chronic diseases;
- •malignant neoplasms, treatment at a time of less than 1 year, autoimmune diseases in the phase of exacerbation;
- •pregnancy/lactation;
- •HIV, Hepatites B/C;
- •active tuberculosis;
- •alcohol use disorder/drug addiction.
结局指标
主要结局
Sensitivity of the diagnosis of bacterial vs viral acute infection
时间窗: 1 month
Sensitivity of the diagnosis of bacterial vs viral acute infection using proposed method
Specificity of the diagnosis of bacterial vs viral acute infection
时间窗: 1 month
Specificity of the diagnosis of bacterial vs viral acute infection using proposed method
次要结局
未报告次要终点
研究者
Andrei Y. Hancharou
Head of the Laboratory for Immunology and Cellular Biotechnology,
The Republican Research and Practical Center for Epidemiology and Microbiology