Effect of Electromyographic Biofeedback on Quadriceps Neuromuscular Function

Screening Subjects will be asked to complete a brief questionnaire about their lower extremity, which will be used to determine if they exhibit any of the exclusionary criteria (administered by the Study Coordinator). This will include past medical history, headache/ migraine history, and any drugs/medications they are currently taking (which might influence the excitability threshold Hoffman reflex (H-reflex) measurement or central activation ratio). Subjects will also undergo quadriceps central activation ratio testing to determine the level of quadriceps inhibition. Upon review of the questionnaire and quadriceps central activation ratio, the study coordinator will determine a subject's eligibility (based on the inclusion and exclusion criteria). We will also collect age, height and weight for all subjects. Participants that qualify will provide written at this time which will be documented by the investigator.

Quadriceps Central Activation Ratio We will use the superimposed burst technique to measure quadriceps muscle strength and to estimate quadriceps activation by calculating the central activation ratio. Subjects will be secured to a chair (Biodex multi-mode dynamometer) with their knees and hips bent to approximately 90 degrees. Subjects will perform a maximal, voluntary isometric knee extension contraction (MVIC) with continuous verbal encouragement from the tester. Once the MVIC reaches a plateau (representing subjects' maximal effort) an electrical stimulus will be manually triggered and delivered directly to the quadriceps through 2 stimulating electrodes which will be secured to the subjects' anterior thigh. The stimulus will cause the quadriceps to twitch resulting in a temporary increase in force production which we will measure. A ratio between the MVIC force and the highest force achieved due to the electrical stimulation will be calculated - this is called the central activation ratio. The electrical stimulus will be delivered manually with a square-wave stimulator in conjunction with a stimulus isolation unit) and will consist of a train of 10 consecutive, square wave pulses of 600 microsecond duration, at an intensity of 125 volts with a carrier frequency of 100 pulses per second. Subjects will have the option of experiencing some "practice" pulses at lower intensity in order to become acclimated to the stimulus and the practice maintaining maximum effort in anticipation of the stimulation.

Randomization Randomization will take place after enrollment and screening take place. The assessor will be blinded in this investigation. Therefore, the clinician responsible for educating patients regarding the exercise protocols will also be responsible for randomization. Randomization will take place on a 1:1 basis. Prior to starting the study, a binary random number generator will be used to generate the randomization scheme. These numbers will then by placed in sealed envelopes and label with individual subject numbers. The randomization envelopes will be stored in a locked drawer and will only be removed by the clinician responsible for randomization. The randomization envelope will then be stored in the participant's research folder.

Quadriceps Hoffmann Reflex We will record muscle reflex activity by delivering a short percutaneous (through the skin) electrical stimulation to femoral nerve located in the inguinal fold. We will measure the reflex response of the quadriceps with surface electromyography. This measure helps to describe the function of a muscle before and after our exercise protocol. Subjects will be positioned comfortably in a lying-down position on a treatment table. Stimuli will be delivered to the femoral nerve by increasing the intensity in small increments with a 10 second rest interval after each stimulus, until the maximum H-reflex amplitude is recorded (Hmax). Then we will increase the stimulus voltage until we obtain a maximum M-wave. The H-reflex represents the proportion of the quadriceps motor neuron pool that is available for voluntary contraction and the M-wave represents the total volume of the quadriceps motor neuron pool and will be used to normalize the H-reflex recordings as H:M ratio. This measurement will be performed bilaterally.

Exercise Intervention Participants in both groups will be instructed on a 14 day exercise (4 supervised session and 10 home sessions) protocol on the day of enrollment. The protocol is comprised of 4 exercises focused on both non-weight bearing and weight bearing quadriceps strengthening. All participants will be asked to complete 3 set of 10 repetitions of isometric quadriceps contractions with a 15 second hold time, supine straight leg raises, body weight squatting, and body weight lunges daily. Participants will be required to complete the single limb exercises on the previously injured limb only. A compliance log will be given to each participant and will be reviewed at each supervised exercise session and at the final study visit. Participants will be instructed to discontinue exercise and contact the principle investigator if they experience knee pain due to the intervention.