Validating the Indicator Amino Acid Oxidation Technique for Muscle Outcomes
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 8
- 试验地点
- 1
- 主要终点
- Phenylalanine excretion (umol/kg/h)
研究概览
简要总结
Consuming dietary protein stimulates whole-body and muscle protein synthesis, the latter of which is typically measured using invasive primed constant infusions of stable isotopes with concurrent muscle biopsies. Alternative non-invasive methodologies have been developed (namely the indicator amino acid oxidation (IAAO) technique) to estimate the impact of protein ingestion on whole-body protein synthesis as a proxy for determining dietary protein requirements. Given that the IAAO technique is based on principles of protein metabolism which occur in the liver, it is unclear how representative the IAAO outcomes of whole-body protein synthesis is to skeletal muscle protein synthesis. Validation of the IAAO technique against gold-standard, biopsy-derived measures of muscle metabolism (i.e., muscle protein synthesis) would assist in mitigating the invasiveness of muscle physiology and nutrition research.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Participants will be aged 18-35 years old
- •Participants will have a BMI within the normal range (i.e., 18.5-24.9) and waist-to-hip circumference ratio of <0.95 for males and <0.8 for circumference for females to ensure homogeneity of the sample population
- •Participants are willing to abide by the compliance rules of this study (e.g., abstain from extraneous physical activity 48h prior to session 4
- •Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants)
- •Physical activity score of ≥24 units as measured by the Godin leisure time exercise questionnaire
排除标准
- •Inability to adhere to any of the compliance rules judged by principal investigator
- •Self-reported regular tobacco or illicit drug use (e.g., growth hormone or testosterone)
- •Current use of hormonal contraceptives
- •Individuals with a history of allergy to local anesthetics (e.g., lidocaine)
结局指标
主要结局
Phenylalanine excretion (umol/kg/h)
时间窗: 6.5 hours
Rate of whole-body phenylalanine excretion following orally ingested amino acid tracer \[1-13C\]Phenylalanine determined from the product of breath 13CO2 enrichment and carbon dioxide production rate (VCO2).
Phenylalanine oxidation (umol/kg/h)
时间窗: 6.5 hours
Rate of whole-body phenylalanine oxidation following orally ingested amino acid tracer \[1-13C\]Phenylalanine determined from the correction of phenylalanine excretion by the phenylalanine precursor enrichment via urine enrichment.
Myofibrillar protein synthesis (%/hr)
时间窗: 6.5 hours
Rate of myofibrillar fractional synthesis is determined by rate of incorporation of orally ingested tracer \[2H5\]Phenylalanine into myofibrillar proteins (isolated from skeletal muscle biopsies) and the enrichment of \[2H5\]Phenylalanine in plasma collected over the 6 hours protein consumption period. Enrichment will be quantified via tandem LC-MS/MS to derive a rate of incorporation over the 6-hour period.
Sarcoplasmic protein synthesis (%/hr)
时间窗: 6.5 hours
Rate of sarcoplasmic fractional synthesis is determined by rate incorporation of orally ingested tracer \[2H5\]Phenylalanine into sarcoplasmic (ie., soluble) proteins (isolated from skeletal muscle biopsies) and the enrichment of \[2H5\]Phenylalanine in plasma collected over the 6 hours protein consumption period. Enrichment will be quantified via tandem LC-MS/MS to derive a rate of incorporation over the 6-hour period.
次要结局
未报告次要终点
研究者
Daniel Moore
Principal Investigator
University of Toronto