Metabolic Profiling of Immune Responses in Immune-Mediated Diseases
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 300
- 试验地点
- 1
- 主要终点
- Change in relative abundance of microbial skin taxa and/or skin metabolites associated with wound healing or immune mediated disorders.
研究概览
简要总结
Background:
The immune system is the part of the body that fights infection. Some people have immune deficiencies that cause skin rashes, make them get sick often with infections, or make it difficult for their skin to heal. Researchers want to learn more to better treat conditions that affect immune response.
Objective:
To learn about how the immune system and skin healing are related to each other.
Eligibility:
People ages 18-75 with primary immune deficiency, eczema, or psoriasis. Healthy volunteers are also needed.
Design:
Participants will be screened with a medical and medicine history and a physical exam. They may take a pregnancy test.
Participants will discuss the medicines or supplements they take as well as skin products they use, such as soaps and lotions.
Participants will have up to 4 skin biopsies taken from the forearm. A needle will inject an anesthetic into the skin where the biopsy will be done. A sharp tool that looks like a tiny cookie cutter will be used to remove a round plug of skin a bit smaller than the tip of a pencil.
Participants will give at least 1 blood sample.
Participants may have optional skin swab collection. A cotton swab will be used to swab the skin on the arm.
Participants may have optional skin tape collection. A sticky strip of tape will be placed on the arm and then removed.
Participants may give leftover samples taken as part of their regular medical care.
Participation will last for about 4 days. Participants will have 2 visits that each last about 1 hour. They may be asked to repeat the study in the future.
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详细描述
Study Description:
This is an exploratory sample collection study enrolling adult healthy volunteers and patients with immune-mediated diseases to elucidate the metabolic signatures associated with immune-mediated diseases. We hypothesize that activation of, and responses to, specific immune pathways will require metabolic changes within cells, serum, and skin.
Following screening and baseline procedures, including blood draw, participants who are able to attend onsite visits will undergo skin biopsies. The skin biopsies will involve up to 4 biopsies: 2 initial punch biopsies 2 mm in diameter, optionally followed by 1 or 2 punch biopsies 3 (+/-10) days later using a 3-mm punch to encompass the initial biopsy site(s), capturing the tissue at 3-14 days of healing. There will be an option for blister induction to maximize keratinocyte collection. Blister induction involves creating 8 blisters on the forearm and removing the tops of the blisters for primary cell culture derivation. In addition, skin swabs and tape strips may be collected to assess for microbial or host markers of epithelial repair. Peripheral blood and functional signatures will be collected with the initial and follow-up biopsies to compare metabolic and functional signatures in peripheral blood cells and serum with those in the skin. Urine may be collected in order to detect metabolites that are not as easily detected in the blood. After day 0 (or 3-14, if the participant has that optional visit), participation in the study will be complete. At-home participants who cannot attend onsite visits may enroll for survey
data collection and optional at-home sample collection (with at least one sample type required). Exposome data will be collected to assess how environmental, occupational, and dietary exposures associate with the results.
Samples and data will be stored for future research use.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 75 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •INCLUSION CRITERIA:
- •In order to be eligible to participate in this study, an individual must meet all of the following criteria:
- •Meets one of the following:
- •Has documentation of PID confirmed by genetic evaluation demonstrating a deleterious variant in the gene (or genes) known to be associated with immune deficiency (confirmed PID); or
- •Has documented variant of undetermined significance in a gene (or genes) that is predicted to be deleterious in immune function by the investigators OR a clinical history of infections which are more frequent, more chronic, or more severe than normal (suspected PID); or
- •Has physician-diagnosed psoriasis; or
- •Has physician-diagnosed AD; or
- •Does not have clinically apparent evidence of any monogenic or digenic immune defect, AD, or psoriasis (healthy volunteers).
- •Aged 18 to 75 years.
- •Willing to allow storage of blood, biopsy tissue, bacterial and fungal cultures, and any other samples collected for future research.
- •Able to provide informed consent.
排除标准
- •An individual who meets any of the following criteria will be excluded from participation in this study:
- •Current or prior (within 3 months) anticoagulant or anti-platelet therapy (other than aspirin or non-steroidal anti-inflammatory drugs).
- •Current or prior (within 3 months) use of immunomodulatory drugs (eg, chemotherapy, steroids), except if approved by the principal investigator.
- •History of keloid formation.
- •Pregnancy, lactating, or breastfeeding.
- •Any condition that, in the opinion of the investigator, contraindicates participation in the study.
- •Co-enrollment guidelines: Participants may be co-enrolled in other studies; however, study staff should be notified of co-enrollment.
研究组 & 干预措施
Healthy volunteers
Control group
Patients with atopic dermatitis
Physician-diagnosed atopic dermatitis
Patients with primary immunodeficiency
Confirmed by genetic diagnosis or suspected by genetic variant of unconfirmed significance and a history consistent with immunodeficiency
Patients with psoriasis
Physician-diagnosed psoriasis
结局指标
主要结局
Change in relative abundance of microbial skin taxa and/or skin metabolites associated with wound healing or immune mediated disorders.
时间窗: Throughout study
Determine whether there are abnormalities in specific tissue repair pathways, such as epithelial to mesenchymal transition (EMT) are associated with immune-mediated disorders.
Fold difference in metabolic pathways associated with immune pathways.
时间窗: Throughout study
Evaluate metabolic profiles in immune activation associated with known or suspected immune-mediated disorders.
Fold differences in metabolic pathways related to wound healing.
时间窗: Throughout study
Determine whether there are abnormalities in specific tissue repair pathways, such as epithelial to mesenchymal transition (EMT) are associated with immune-mediated disorders.
次要结局
未报告次要终点