NCT01450852已完成不适用

Strength Training Induced Alterations in Markers of Immune Function

Louisiana State University Health Sciences Center in New Orleans1 个研究点分布在 1 个国家目标入组 42 人开始时间: 2010年1月1日最近更新: 2011年10月12日

适应症

干预措施

试验速览

阶段: 不适用
状态: 已完成
发起方: Louisiana State University Health Sciences Center in New Orleans
入组人数: 42
试验地点: 1
主要终点: Melanocortin 1 Receptor Expression on Monocytes

研究概览研究设计入排标准研究组 & 干预措施结局指标研究者研究点记录与标识符相似试验

研究概览

简要总结

Exercise has been used to help prevent or slow the progression of inflammation-related disease; however, the mechanism by which this activity may lower concentrations of inflammatory markers remains unclear. The melanocortin receptors 1,3 and 5 (MC1R, MC3R and MC5R) have been shown to function in an anti-inflammatory manner and have the potential to mediate the positive immune adaptations associated with regular physical activity.

Preliminary data suggest that MC3R gene expression increases in whole blood after chronic exercise training. The primary aim of the current study is to explore whether this change in gene expression translates into alterations in MC1R, MC3R, or MC5R monocyte surface expression. The secondary aim is to examine the relationship between surface expression of these receptors and circulating inflammatory profiles.

The investigators will recruit 42 untrained, healthy males and females aged 18-35 yrs. Half of the group will be placed on an exercise program for 15 weeks. The other half will serve as untrained control subjects. In addition to basic anthropometric measures, the investigators will measure concentrations of inflammatory and anti-inflammatory cytokines (ELISA) and cell surface expression of MC1R, MC3R, and MC5R on monocytes (flow cytometry).

详细描述

Abstract:

Chronic exercise reduces inflammation, but the mechanisms involved are unclear. Recent studies have shown that stimulation of melanocortin 1 and 3 receptors (MC1R and MC3R) on immune cells increases anti-inflammatory cytokine production. PURPOSE: To examine the influence of 12 weeks of resistance training (RT) on body composition, monocyte cell-surface expression of MC1R and MC3R and circulating markers of inflammation. METHODS: Healthy, active males and females (age 20-27 yr) were recruited into a RT group (RE; n = 23) and an active control group (AC; n = 19). RE completed 12 weeks of progressive, periodized RT 3d/wk while AC maintained normal activity habits. Measures of body composition (DXA) were taken and blood was collected prior to (PRE) and following the intervention period (POST). Blood samples were analyzed for monocyte cell-surface expression MC1R, MC3R, MC5R and C-reactive protein (CRP) and the plasma cytokines interleukin 6 (IL-6) and interleukin 10 (IL-10) using flow cytometry and ELISAs respectively.

研究设计

研究类型: Interventional
分配方式: Non Randomized
干预模型: Parallel
主要目的: Prevention
盲法: None

入排标准

年龄范围: 18 Years 至 30 Years（Adult）
性别: All
接受健康志愿者: 是

入选标准

•All Participants Must be Healthy
•The Strength Training Group Members Must Be Approved For Participation by a Licensed MD

排除标准

•Females may not be pregnant
•Unhealthy participants (those with existing conditions)

研究组 & 干预措施

Strength Training

Experimental

The strength training group completed 12 weeks of progressive, periodized resistance training 3d/wk.

干预措施: Strength Training (Behavioral)

Active Control Group

No Intervention

The active control group was instructed to maintain normal activity and eating habits. They participated in all pre and post intervention measures.

结局指标

主要结局

Melanocortin 1 Receptor Expression on Monocytes

时间窗: Before and After 12 Week of Intervention Period

Blood samples were analyzed for monocyte cell-surface expression of the melanocortin 1 receptor using flow cytometry. Pre samples were collected in Jan. 2010 and post samples were collected in May 2010 from all subjects.

Plasma CRP