Effect of Face Masks on the Microbial Communities of the Skin and Upper Airways and the Optimalisation of Microbiome Analysis
试验速览
- 阶段
- 不适用
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- Optimalization of microbiome analysis of skin and upper airway samples
研究概览
简要总结
The goal of this study is to optimize two methods for microbiome analysis of skin and upper airway samples (16S amplicon sequencing and Shotgun sequencing). In addition, the researchers will investigate whether there is an effect of wearing a face mask on the microbial communities of the skin and upper airways. The volunteers will self-collect swabs of the skin and upper airways (nose and/or throat). Bacterial DNA and/or total RNA will be isolated and the microbial communities will be investigated with 16S amplicon sequencing and shotgun metagenomic sequencing.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Adult age 18 at the start of the study
排除标准
- •Pregnant women
结局指标
主要结局
Optimalization of microbiome analysis of skin and upper airway samples
时间窗: Start
16S rRNA sequencing, shotgun metagenomic sequencing
Change from baseline in microbiome composition of the skin and upper airways after wearing a face mask
时间窗: Start, 4 hours
16S rRNA sequencing
次要结局
未报告次要终点
研究者
Sarah Lebeer
Principal Investigator
University Hospital, Antwerp