Impact of Exercise Intensity on the Time-course of Mitochondrial Biogenic Gene and Protein Expression in Human Skeletal Muscle.
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 10
- 试验地点
- 2
- 主要终点
- Change in peroxisome proliferator activated receptor (PPAR) gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression
研究概览
简要总结
This study examines the impact of exercise intensity on the 12-hour time-course of mitochondrial biogenic gene and protein expression in human skeletal muscle. Briefly, participants will perform two experimental sessions involving an acute bout of work-matched low- or high-intensity interval cycling exercise in a randomized crossover fashion, two weeks apart. Skeletal muscle biopsies will be obtained from the vastus lateralis (3 from each leg) before and 1, 3, 6, 9, and 12 hours post-exercise. Changes in the expression of various transcription factors and mitochondrial proteins will be examined at the mRNA and protein level to determine the time-course of changes in these factors differs between exercise protocols of different intensities.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Double (Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •recreationally active (no more than three hours of structured weekly physical activity)
- •not currently involved in a systematic training program for improving cardiorespiratory fitness, muscular strength, and/or sport-specific skills
- •body mass index < 30 kg/m2
排除标准
- •presence of cardiometabolic disease
- •taking regular prescription medication for the treatment of cardiometabolic disease
- •current cigarette smoker
结局指标
主要结局
Change in peroxisome proliferator activated receptor (PPAR) gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression
时间窗: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
PGC-1α gene expression (indirect indicator of PGC-1α activity). Transcriptional co-activator involved in the control of mitochondrial biogenesis.
次要结局
- Change in PGC-1α protein content(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in Nrf2 protein content(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in LRP130 protein content(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)
- Change in COXI mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in NRF-2 mRNA expression(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)
- Change in nuclear factor-erythroid 2 like 2 (Nrf2) mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in NRF-1 protein content(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in ND4 mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in PPAR-beta mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in estrogen related receptor (ERR)-gamma mRNA expression(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)
- Change in leucine-rich pentatricopeptide repeat containing protein 130 (LRP130) mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in mitochondrial transcription factor A (TFAM) mRNA expression(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)
- Change in TFAM protein content(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)
- Change in p53 mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in cytochrome c oxidase subunit 4 (COXIV) mRNA expression.(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in COXII mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in nicotinamide adenine dinucleotide (NADH) subunit 1 (ND1) mRNA expression(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)
- Change in citrate synthase (CS) mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in nuclear respiratory factor-1 (NRF-1) mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in mitochondrial transcription factor B1 (TFB1M) mRNA expression(before exercise to 1, 3, 6, 9, and 12 hours post-exercise)
- Change in mitochondrial transcription factor B2 (TFB2M) mRNA expression(before exercise and 1, 3, 6, 9, and 12 hours post-exercise)
研究者
Brendon Gurd, PhD
Associate Professor
Queen's University