Genetic Variation in Platelet Aggregation
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 117
- 试验地点
- 1
- 主要终点
- LogEC50 Platelet Aggregation
研究概览
简要总结
The aim of the study is to test whether genetic variation in the alpha 2A adrenergic receptor affects diurnal variation in platelet aggregation.
详细描述
There is a marked diurnal fluctuation in the occurrence of acute myocardial infarction and sudden death, with peak incidences occurring in the early morning. Platelet aggregation has also been shown to increase in the early morning. The investigators will test the hypothesis that alpha 2a-adrenergic receptor (ADRA2A) genetic variation, specifically haplotype 4, affects platelet aggregation. The investigators will compare diurnal platelet aggregation in haplotype 4 subjects with subjects in the other haplotype families. In addition, the investigators will compare platelet aggregation after the cold pressor test in haplotype 4 with the other haplotype families. If there are no differences among haplotypes, then the haplotypes will be combined to analyze.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Men and women aged 18 - 45 years inclusive.
- •Women of the above age will be studied between day 1 and day 14 of a normal menstrual cycle.
- •Subjects must be willing to give informed consent for the study and able to adhere to the study diet and study procedures.
- •Subjects and immediate extended family up till grandparents will be Caucasians or African Americans only.
- •Subjects will be free of any clinically significant disease.
- •Clinical laboratory test (CBC, blood chemistry) will be within acceptable limits.
排除标准
- •Subjects who have taken any antiplatelet, anticoagulant or procoagulant medicines within the last three weeks preceding the study.
- •Subjects who have taken medications (including over the counter medications) other than oral contraceptives in the past two weeks.
- •Subjects who smoke or have smoked in the past 3 months.
- •Subjects who are presently or were formerly a narcotic addict or alcoholic.
- •Females with a positive pregnancy test.
- •Females who are breast feeding.
结局指标
主要结局
LogEC50 Platelet Aggregation
时间窗: at 6am and 930am
EC50 represents the epinephrine concentration that produced 50% of maximal platelet aggregation (representing sensitivity to epinephrine) EC50 values were not normally distributed and were log-transformed for analyses.
次要结局
- Percentage, Adenosine Diphosphate (ADP) Induced Platelet Aggregation(at 6am and 930am)
- Percentage, Collagen Induced Platelet Aggregation(at 6am and 930am)
研究者
C. Michael Stein
Dan May Professor of Medicine and Pharmacology
Vanderbilt University