Effect of Dietary DHA Supplementation on Sperm Quality.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 120
- 试验地点
- 2
- 主要终点
- sperm concentration
研究概览
简要总结
The aim of this study is evaluated the effect of dietetic supplementation with DHA on human sperm quality, antioxidant capacity and sperm DNA damage.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Double (Participant, Investigator)
入排标准
- 性别
- Male
- 接受健康志愿者
- 否
入选标准
- •Diagnosis of male factor.
排除标准
- •Metabolic and genetic diseases.
- •Oncological patients.
- •Patients with anticoagulants treatment.
结局指标
主要结局
sperm concentration
时间窗: 5 weeks
million of spermatozoa per milimitre
Sperm motility
时间窗: 5 weeks
Percentage of motile sperm.
Sperm morphology
时间窗: 5 weeks
Percentage of sperm with normal morphology
Total capacity antioxidant
时间窗: 5 weeks
Total antioxidant capacity (TAC)is a colorimetric assay that measure the combined antioxidant activities of all its non enzymatic constituents in seminal plasma.
Sperm DNA damage.
时间窗: 5 weeks
DNA fragmentation analysis was evaluated by TUNEL test and measured by flow cytometry
Fatty acids measure.
时间窗: 5 weeks
Fatty acid analysis in seminal plasma and spermatozoa were evaluated by gas chromatography.The percentage of each fatty acid class was expressed as percentage of total fatty acids.
次要结局
- Sperm in ejaculate(5 weeks)
- Volume of ejaculate.(5 weeks)
- Sperm viability(5 weeks)