Oxidative Stress in Testicular Tissue Before and After Cryopreservation
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 58
- 试验地点
- 1
- 主要终点
- Measurement of the level of oxidative stress that is caused by cryopreservation of testicular tissue.
研究概览
简要总结
The absence of sperms in ejaculated semen, is the most severe form of male-factor infertility and is present in approximately 5 % of all investigated infertile couples.
详细描述
Obstructive azoospermia is defined as the absence of spermatozoa in the ejaculate despite normal spermatogenesis.Infection, Iatrogenic injury, Vasectomy and congenital atresia are the possible causes of it. Non obstructive or functional azoospermia results from testicular failure, which is the absence or inadequate production of fully developed spermatozoa within the testes.
Etiologies for testicular failure include genetic disorders such as sexual chromosomal abnormalities ; translocations and microdeletions of the Y chromosome; cryptorchidism; testicular torsion; radiation and toxins.
The advent of intra-cytoplasmic sperm injection has transformed the treatment of this type of severe male-factor infertility.
Surgical techniques for sperm retrieval:Testicular sperm aspiration: In men with obstructive azoospermia ,testicular sperm aspiration invariably generates enough sperm for intra-cytoplasmic sperm injection.
Testicular sperm extraction:This conventional method of surgical sperm extraction generally offers the best chance of retrieving spermatozoa, especially in functional cases and when guided by surgical microscope. However, it can be done by simple open conventional biopsy and closed surgical extraction resembling Trucut biopsy using a special cannula.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 14 Years 至 80 Years（Child, Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 否
入选标准
- •Men with azoospermia at reproductive age .
排除标准
- •Pyospermia.
- •Previous trial of cryopreservation.
- •Systemic diseases.
- •Cryptorchidism history.
结局指标
主要结局
Measurement of the level of oxidative stress that is caused by cryopreservation of testicular tissue.
时间窗: 3 years
Evaluation of oxidative stress caused by cryopreservation of testicular tissue by measurement of : 1. Catalase activity as one indicator of antioxidants by colorimetric method. 2. Malondialdehyde level as one indicator of lipid peroxidation by colorimetric method.
次要结局
未报告次要终点
研究者
Aya Hasan
Resident in dermatology department
Assiut University