Glycocalyx Damage During Normothermic Graft Perfusion in Orthotopic Liver Transplantation - A Pilot Study
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 30
- 试验地点
- 2
- 主要终点
- Syndecan 1 level during normothermic machine perfusion
研究概览
简要总结
An organ intended for transplantation is normally stored on ice (cold storage, CS) after explantation from the donor. During this storage process, damage to the endothelial glycocalyx occurs. It is known from numerous studies that the integrity of the endothelial glycocalyx is necessary for organ function. Normothermic machine perfusion is an alternative storage method for explanted livers, where the graft is perfused with warm blood. This study aims to clarify the influence of normothermic machine perfusion on endothelial glycocalyx damage of the graft.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •First deceased donor liver transplantation receiving a graft after normothermic machine perfusion
- •Recipient age >18 years
- •Written informed consent
排除标准
- •Age < 18 years
研究组 & 干预措施
Normothermic Machine Perfusion
Blood sampling at 5 time points after transplantation Perfusate collection at 3 time points during normothermic machine perfusion prior to transplantation
干预措施: Heparansulfate and Syndecan 1 (Diagnostic Test)
结局指标
主要结局
Syndecan 1 level during normothermic machine perfusion
时间窗: 24 hours
Syndecan 1 level at the end of normothermic perfusion compared to the beginning of perfusion
Heparansulfate level during normothermic machine perfusion
时间窗: 24 hours
Heparansulfate level at the end of normothermic perfusion compared to the beginning of perfusion
次要结局
- Syndecan 1 after transplantation(72 hours)
- Heparansulfate level after transplantation(72 hours)