Effects of a High-fat Meal on Exercise-mediated Sympatholysis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 15
- 试验地点
- 1
- 主要终点
- Systolic and Diastolic Blood Pressure
研究概览
简要总结
The goal of this trial is to examine the effects of a single high-fat meal with or without the co-ingestion of dietary nitrate on exercise-mediated sympatholysis. The main questions it aims to answer are:
- What are the mechanisms responsible for reduced exercise-mediated sympatholysis following a high-fat meal
- Can dietary nitrate prevent the declines in exercise-mediated sympatholysis
Participants will be asked to complete three study visits in a randomized order:
- Low-fat meal
- High-fat meal
- High-fat meal plus dietary nitrate
Exercise-mediated sympatholysis will be measured using Doppler ultrasound via a protocol involving rhythmic handgrip exercise and a lower body negative pressure stimulus. Sympatholysis will be assessed before each meal, and at 1, 2, and 3 hours post-prandial.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Double (Participant, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •18-30 years of age
- •Healthy, free of known cardiovascular, metabolic, or musculoskeletal disease
排除标准
- •Diagnosed cardiovascular or metabolic disease, injury preventing handgrip exercise, prescription of chronic medications other than oral contraceptives.
- •Inability to abstain from drugs (except oral contraceptives), caffeine, alcohol, strenuous physical activity, mouthwash, and multivitamins prior to study
研究组 & 干预措施
High-fat meal with Beetroot Drink
2 sausage egg McMuffins and a hashbrown from McDonalds and 10g of beetroot powder mixed in 250 mL cold tap water drink
干预措施: Beetroot drink (Dietary Supplement)
High-fat meal with Placebo Drink
2 sausage egg McMuffins and a hashbrown from McDonalds and Mio water flavouring in 250 mL cold tap water. Trace amounts of sugar and sodium were mixed to match the beetroot supplement
干预措施: Placebo (Dietary Supplement)
Low-fat meal control
Kelloggs cornflakes (110g), greek yogurt (400g), skim milk (500 mL), and orange juice (250 mL). Energy-matched to the high-fat meal
干预措施: Control (Dietary Supplement)
结局指标
主要结局
Systolic and Diastolic Blood Pressure
时间窗: Before intervention and 1, 2, 3 hours post-intervention
finger plethysmography
Forearm blood flow
时间窗: Before intervention and 1, 2, 3 hours post-intervention
Duplex ultrasound of brachial artery
Forearm vascular conductance
时间窗: Before intervention and 1, 2, 3 hours post-intervention
Duplex ultrasound of brachial artery
次要结局
- Plasma levels of Nitrate/Nitrite concentration(Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date)
- Blood Glucose concentrations(Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood analyzed immediately after collection at each time point)
- Plasma levels of Triglycerides(Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date)
- Plasma levels of Norepinephrine(Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date)
研究者
Philip Millar
Professor
University of Guelph