Changes in Alveolar Macrophage Function During Acute Lung Injury
试验速览
- 阶段
- 不适用
- 入组人数
- 25
- 主要终点
- alveolar macrophages (AMs) phagocytic index
研究概览
简要总结
In this study, the investigators aim to observe the change in alveolar macrophage function during acute lung injury.
详细描述
Alveolar macrophages are tissue-resident or recruited cells with key functions in recognition of pathogens, initiation of host defensive ang protective inflammation,and in clearance of pathogens from the airways. However, macrophages are also responsible for efficient clearance of apoptotic neutrophils, termed efferocytosis, which is a prerequisite for the timely resolution of inflammation. In this study, the investigators aim to observe the change in alveolar macrophage function during acute lung injury.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Patients in ICU with diagnosed ALI under mechanical ventilation
排除标准
- •Patients with cardiac dysfunction, using steroid drugs or other drugs can that affect efferocytosis
结局指标
主要结局
alveolar macrophages (AMs) phagocytic index
时间窗: 7 days after admission
AMs will be collected and cultured in vitro. Apoptotic neutrophils will then be cocultured with AMs for 1 hour. To measure phagocytosis of apoptotic neutrophils, AMs were further processed and phagocytosed apoptotic neutrophils were detected by confocal fluorescence microscopy as described below under Cell staining and confocal microscopy. The phagocytic index was calculated according to the following formula: phagocytic index = (total number of engulfed cells/total number of counted macrophages) × (number of macrophages containing engulfed cells/total number of counted macrophages) × 100.
次要结局
- 30 days mortality(30 days after admission)