The Influence of Citric Acid Bone Surface Etching and Bone Substitute Intra-osseous Defect Fill on Crevicular Fluid BMP-2 Release Profile (Randomized Clinical Trial)
试验速览
- 阶段
- 4 期
- 状态
- 已完成
- 入组人数
- 20
- 主要终点
- Bone Morphogenic protein 2
研究概览
简要总结
This study evaluates the effect of citric acid on bone surface of infrabony defects when used for 30 seconds at ph 1 and 50% concentration before the application of bovine derived xenograft to fill the defect, Bone Morphogenic protein 2 (BMP-2) marker was evaluated in 5 different days during the first month after surgery and clinical and radiographic parameters were reassessed after 6 months
详细描述
Citric acid have been widely used to treat root surfaces of periodontally affected teeth in order to render it biologically suitable for fibroblast reattachment. however, systematic reviews showed no clinical significance when it was used as a root surface conditioner. Recently it was found in experimental studies that when citric acid was used on bone surface for a brief period, it increased bone formation and improved consolidation of grafted bone to bone bed. In this study infrabony defects were treated with citric acid PH 1 and 50% concentration for 30 seconds before washing it off and then application of bovine derived xenograft was done to fill the defect. BMP-2 marker was evaluated at days 1,3,7,14 and 30 by taking a sample using perio-paper from the gingival crevicular fluid (GCF) and clinical and radiographic parameters were taken after 6 months of follow-up
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 20 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Both genders aged between 32-60 years.
- •Selected patient with a single site of:
- •Two walled or three walled infra-bony defect.
- •The bone defect should be at least 3 mm in depth from the crest of the alveolar bone to the base of the defect.
- •Pocket depth of more than or equal to 5 mm.
- •Clinical attachment loss equal or more than 3mm
- •Patients ready to comply with oral hygiene measures.
排除标准
- •Any systemic disease that contra-indicates periodontal surgery or may affect healing.
- •Pregnant females
- •Drug abusers
研究组 & 干预措施
Citric acid
Citric acid PH1 and concentration 50% was used for 30 seconds on bone surface before washing it off and filling the defect with xenograft
干预措施: Citric acid (Drug)
Control
Control test
干预措施: Control Test (Drug)
结局指标
主要结局
Bone Morphogenic protein 2
时间窗: 30 days
The level of Bone morphogenic protein 2 was measured in the gingival crevicular fluid using a perio-paper then the marker level was determined using an Enzyme linked immunosorbent kit (ELISA kit)
次要结局
- Gingival Index(6 months)
- Plaque index(6 months)
- Probing depth(6 months)
- Clinical attachment level(6 months)
- Radiographic bone fill(6 months)
研究者
Moustafa Mukhtar
Teaching assistant
Ain Shams University