Application of multimodal Raman tweezers to study biochemical variations in human erythrocytes due to physiological aging of individuals
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 192
- 试验地点
- 2
- 主要终点
- These are the prominent measures used to study the usefulness, associations between the prominent variables, and groups or themes generated at the end of the study.
研究概览
简要总结
This study uses Raman spectroscopy with optical tweezers to analyze red blood cells (RBCs) from individuals of different age groups, the study involves 192 healthy participants across different age groups: 60 young (20–39), 60 middle-aged (40–59), and 60 elderly (60+), plus 12 volunteers (6 young and 6 middle-aged) who provide blood samples every three months for three years with routine tests. The Leftover blood samples will be transported from the clinical lab to the experimental lab under cold chain conditions to record Raman spectra from 10–15 individual RBCs per participant. Using different laser focal spots(point, donut, and line), the study aims to detect age-related biochemical changes in RBCs. Statistical analysis will classify these changes and relate them to clinical data, preserving the cells’ natural state without invasive procedures. This approach will create a detailed spectral dataset to identify markers of cellular aging and link them to biochemical changes in RBCs. It also offers new insights into how aging affects RBC structure and function, with the potential to advance future blood research using Raman tweezer technology.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 盲法
- None
入排标准
- 年龄范围
- 20.00 Year(s) 至 60.00 Year(s)（—）
- 性别
- All
入选标准
- •Age Range: To facilitate a comparative analysis of aging biomarkers, participants will be selected from distinct Age groups, including young adults (20-39 years), middle-aged individuals (40-59 years), and the elderly (60+ years).
- •We will consider 30 men and 30 women in each group of 60 participants above.
- •Hemoglobin level: Participants with a minimum hemoglobin level requirement.
- •Male: 13.2 to 16.6 grams per deciliter (g/dL) Female: 11 to 14 g/dL (on average).
排除标准
- •Participants undergoing recent medical treatments (e.g., chemotherapy, immunosuppressive therapy, blood transfusions, or vaccinations), those with blood disorders (like anemia or HIV), diabetic patients, and individuals with genetic syndromes affecting blood will be excluded.
结局指标
主要结局
These are the prominent measures used to study the usefulness, associations between the prominent variables, and groups or themes generated at the end of the study.
时间窗: 3 years
1. The study will generate a comprehensive Raman spectral dataset from RBCs of individuals spanning different ages. The research is expected to elucidate specific markers indicative of cellular aging by correlating these spectral variations with age.
时间窗: 3 years
2. The analysis will provide insights into how Raman spectral variations relate to biochemical parameters for aging. The study will deepen our understanding of how human aging impacts the functionality and detection of molecular and structural alterations in RBCs.
时间窗: 3 years
3. Using Raman tweezer setups is a new way to study how individual cells behave as they age. The success of this technology could lead to more advanced experimental designs in future blood-related research.
时间窗: 3 years
次要结局
未报告次要终点
研究者
Vidya Rastapur
Manipal Institute of Applied Physics, MAHE, Manipal