Association of serum SIRT-1 level and PCSK9 gene polymorphism in coronary artery disease with metabolic syndrome
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- association of serum SIRT1 and metabolic syndrome in patients of coronary artery disease
研究概览
简要总结
The present study will be conducted with the primary objective of discovering association of serum SIRT1 and PCSK9 gene polymorphism with metabolic syndrome in patients of coronary artery disease The subjects(60) will be the diagnosed cases of coronary artery disease and divided into two groups one with metabolic syndrome and the other without metabolic syndrome based on the National Cholestrol Education Program Adult Treatment Panel III definition
After informed consent 5 ml of venous blood sample will be collected in the plain vial 3ml and EDTA vial 2ml Serum SIRT1 level will be estimated by ELISA technique following the protocol as per manual The genotyping for PCSK9 gene polymorphism will be done using Allele Specific Oligonucleotide Polymerase chain reaction ASO PCR and RFLP
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 40.00 Year(s) 至 65.00 Year(s)（—）
- 性别
- All
入选标准
- •Cases of stable and unstable angina, and follow cases of myocardial infarction.
排除标准
- •All other cardiac disorders.
结局指标
主要结局
association of serum SIRT1 and metabolic syndrome in patients of coronary artery disease
时间窗: 4 weeks
次要结局
- association between pcsk9 gene polymorphism & serum SIRT1 in patients of coronary artery disease with metabolic syndrome(4 weeks)
研究者
Dr Anwar Pervez
Maulana Azad Medical college