NCT03984383撤回不适用
Regulation of IL33, Endocan and Their Targets in Primary Human Endothelial Cells
University Hospital, Lille1 个研究点 分布在 1 个国家开始时间: 2021年11月8日最近更新:
适应症
试验速览
- 阶段
- 不适用
- 状态
- 撤回
- 发起方
- 试验地点
- 1
- 主要终点
- change in the level of messenger RNA expressed as IL-33 / GAPDH ratio between stimulated and unstimulated primary human lung endothelial cells.
研究概览
简要总结
The study aims to investigate the inflammatory response of endothelial cells to various stimulations, in particular the production of IL33 and of endocan in response to allergens, agonists of microorganisms and pollutants.
For that purpose, this project attempts to set up a biological collection of lung and umbilical cord endothelial cells.
Lung endothelial cells are resected from a surgical specimen, resulting from a lung cancer surgery.
Umbilical cord-derived endothelial cells are taken from the umbilical cord collected during the delivery.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Age > 18 years
- •signed consent
- •Pregnant woman for umbilical cord-derive-endothelial cells
排除标准
- •Age < 18 years
- •Refusal to participate to the study
- •Endothelial cord-drived endothelial cells :
- •significant materno-foetal disorder (for example: meconium-stained amniotic fluid, chorioamnionitis, placental thrombosis, eclampsia etc.)
- •infection for HIV, VHB, VHC
- •unknown status for HIV, VHB, VHC the day of the childbirth.
结局指标
主要结局
change in the level of messenger RNA expressed as IL-33 / GAPDH ratio between stimulated and unstimulated primary human lung endothelial cells.
时间窗: change from Baseline at 48 hours after stimulation
次要结局
- change in IL33 protein concentration between stimulated and unstimulated primary human lung endothelial cells.(change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation)
- change in the level of messenger RNA expressed as endocan / GAPDH ratio between stimulated and unstimulated primary human umbilical cord-derived endothelial cells.(change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation)
- change in the level of messenger RNA expressed as IL-33 / GAPDH ratio between stimulated and unstimulated primary human umbilical cord-derived endothelial cells.(change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation)
- change in the level of messenger RNA expressed as endocan / GAPDH ratio between stimulated and unstimulated primary human lung endothelial cells.(change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation)
- change in the level of messenger RNA expressed as IL-33 / GAPDH ratio between stimulated and unstimulated primary human lung endothelial cells.(change from Baseline at 4 hours, 12h ours, 24 hours after stimulation)
- change in IL33 protein concentration between stimulated and unstimulated primary human umbilical cord-derived endothelial cells.(change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation)
- change in endocan protein concentration between stimulated and unstimulated primary human lung endothelial cells.(change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation)
- change in endocan protein concentration between stimulated and unstimulated primary human umbilical cord-derived endothelial cells.(change from Baseline at 4 hours, 12h ours, 24 hours and 48 hours after stimulation)
研究者
研究点 (1)
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