Human Requirements for the Nutrient Choline
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 43
- 试验地点
- 2
- 主要终点
- Evidence of liver or muscle dysfunction (based on elevations in CPK, AST, ALT), or increased liver fat (measured by liver MRI)
研究概览
简要总结
The purpose of this study is to increase our understanding of how much choline humans need to get from their diet. Choline is an essential nutrient found in many foods, including eggs and milk. In addition to dietary sources, choline can be made in the liver. Choline is important in making membranes or wrappers for all the cells in the body and for making chemicals that allow nerve cells to work properly. In a previous study we found that the dietary requirement for choline varies greatly from person to person. This was caused, in part, by how much estrogen a person has and their genetic makeup. We are conducting this study to explore how estrogen levels and specific differences in genes influence choline requirements so that we can refine the dietary recommendations for this nutrient.
详细描述
Choline is an essential nutrient essential used for the structural integrity and signaling functions of cell membranes, cholinergic neurotransmission, and lipid transport/metabolism. Choline is obtained from the diet and from endogenous biosynthesis catalyzed by the enzyme phosphatidylethanolamine N-methyltransferase (PEMT). The major premise for this proposal is that humans require a dietary source of choline and that this requirement has significant individual variation and is modulated by estrogen and common genetic polymorphisms. The promoter of the PEMT gene is estrogen responsive, and we hypothesize that estrogen status influences the dietary requirement for choline. We identified other common single nucleotide polymorphisms (SNPs) that increase or decrease the likelihood that a human will develop organ dysfunction when fed a low choline diet. Experiments are proposed that will refine our understanding of estrogen-mediated induction of the PEMT promoter; determine whether postmenopausal women treated with estrogen have a decreased susceptibility to developing organ dysfunction associated with choline deficiency; determine the prevalence of SNPs that increase susceptibility to choline deficiency in the population and examine dietary choline requirements in humans with these SNPs.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 85 Years（Adult, Older Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Non-smoker
- •BMI between 18 and 34
- •Normal mammogram in last 12 months (post-menopausal women only)
排除标准
- •Hormone or estrogen therapy
- •Allergic to soy, eggs, wheat
- •History of breast, uterine, or other estrogen-dependent cancer
- •Liver or kidney problems
- •History of circulation, bleeding, or blood-clotting disorder
- •Anemia or evidence of iron overload
- •Hyperthyroidism, neurological disorder, or autoimmune disease
- •Diabetes controlled by insulin
- •Positive serology for HIV or Hepatitis B or C
- •Alcohol or illegal drug misuse/abuse
- •Pacemaker, aneurysm clip, cardiac heart valve, mechanical devices/implants
- •Other metal in body (i.e. injured by a BB, shrapnel, or metallic object)
结局指标
主要结局
Evidence of liver or muscle dysfunction (based on elevations in CPK, AST, ALT), or increased liver fat (measured by liver MRI)
时间窗: Labs measured every 3-4 days throughout 62-day trial. Liver MRI performed on study days 1, 10, 31, 52, 62.
次要结局
未报告次要终点
研究者
Steven Zeisel
Professor of Nutrition and Pediatrics, Director, UNC Nutrition Research Institute
University of North Carolina, Chapel Hill