Expression of Wnt5a and WNT Coreceptor (LRP5) Levels in Stage III Periodontitis Patients
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 40
- 试验地点
- 1
- 主要终点
- Wnt5a
研究概览
简要总结
Objectives: Wnt5a is a secreted Wnt ligand that plays an important role in cellular homeostasis. Wnt interaction with coreceptor low-density lipoprotein receptor-related protein 5 (LRP5) induces the proliferation of osteoblasts and prolongs their functional life. The aim of this study is evaluating the levels of Wnt5a and LRP5 in serum and saliva samples and relationship between clinical periodontal parameters.
Method: Saliva and serum samples were collected from 20 systemically healthy patients with stage III periodontitis and 20 periodontally healthy controls. Wnt5a and LRP5 levels were measured using enzyme-linked immunosorbent assay (ELISA). Clinical periodontal parameters including plaque index (PI), probing pocket depth (PPD), bleeding on probing (BOP), and clinical attachment level (CAL) were recorded.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 70 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •>18 years old,
- •At least 16 permanent teeth except 3rd molars,
- •Individuals who do not use orthodontic appliances,
- •Individuals who are not pregnant or lactating,
- •Individuals without any systemic disease that may affect periodontal health,
- •Systemically healthy individuals,
- •Individuals who have not used anti-inflammatory and/or anti-microbial drugs in the last 6 months,
- •Individuals who have not received periodontal treatment in the last 1 year
排除标准
- •Pregnant/lactating individuals,
- •Individuals who have used anti-inflammatory and/or anti-microbial drugs in the last 6 months,
- •Individuals who have undergone periodontal treatment in the last 1 year,
- •Individuals with psychiatric illness,
- •Individuals with any oral infection,
- •Individuals with <16 teeth, excluding molars,
- •Individuals with alcohol dependence,
- •Individuals with active infectious disease (acute hepatitis, AIDS, tuberculosis), cancer or any systemic condition that may affect periodontal tissues,
- •Individuals receiving treatment with drugs known to affect periodontal tissues (phenytoin, cyclosporine A, calcium channel blockers)
结局指标
主要结局
Wnt5a
时间窗: 1 month
Wnt5a is synthesized by activated antigen-presenting cells and its expression is induced by mycobacterial cell wall components and endotoxin in human antigen-presenting cells, and Wnt5a signaling is required for the overall inflammatory response of human macrophages during sepsis.The levels of Wnt5a in both serum and saliva samples were determined using commercially available ELISA kits. All assays were conducted according to the manufacturers' instructions.
LRP5
时间窗: 1 month
Low-density lipoprotein (LDL) receptor-related protein 5 (LRP5) is a WNT coreceptor in the LRP superfamily involved in a wide range of biological processes including endocytosis, cellular communication, lipid homeostasis and embryonic development. The levels of LRP5 in both serum and saliva samples were determined using commercially available ELISA kits. All assays were conducted according to the manufacturers' instructions.
次要结局
未报告次要终点
研究者
Zulal Deniz Guner
Sub-Investigator
Ankara University