Effect of Exercise on MiR-126 in Individuals With Prediabetes: A Randomized Controlled Trial
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 64
- 试验地点
- 1
- 主要终点
- Delta CT mir-126
研究概览
简要总结
This study aims to investigate the effect of a structured exercise program on microRNA-126 (miR-126) expression in individuals with prediabetes.
详细描述
This study aims to investigate the effect of a structured exercise program on microRNA-126 (miR-126) expression in individuals with prediabetes. The hypothesis of our study is that regular aerobic exercise will increase miR-126 expression. Determining the effect of exercise on miR-126 in individuals with prediabetes will contribute to the elucidation of the pathways through which exercise is effective in prediabetes via epigenetic mechanisms.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Prevention
- 盲法
- Double (Care Provider, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Individuals who were newly diagnosed with prediabetes according to the Oral Glucose Tolerance Test (OGTT) and/or HbA1C criteria
- •between the ages of 18-60
- •were physically inactive (Level 1 according to the International Physical Activity Questionnaire)
- •were motivated to exercise
- •Individuals who had no obstacle to exercise as a result of the evaluation made by a cardiologist (ECG, exercise ECG test, etc.)
- •and who did not show a change in body weight of more than ±2.5 kg in the last six months were included in the study.
排除标准
- •presence of serious medical conditions (e.g. advanced cancer, major neurological or endocrine disorders, respiratory failure)
- •current cardiovascular disease, BMI <19 kg/m²
- •history of metformin use within the last 6 months
- •receiving hormone replacement therapy
- •life expectancy less than one year
- •HIV positivity
- •substance use
- •or orthopedic and cognitive disorders that may prevent exercise
研究组 & 干预措施
Exercise
Individuals in the intervention group were included in a planned aerobic exercise program for 12 weeks.This program was carried out under the supervision of specialist doctors, with the help of sports trainers and physiotherapists, and participants were given 60 minutes of moderate-intensity (50-70% of their maximum heart rate) aerobic exercise three days a week.
干预措施: Exercise (Behavioral)
Control
No intervention
结局指标
主要结局
Delta CT mir-126
时间窗: 1 week
Ct (cycle threshold) values for miR-126 and miR-16-5p were used for analysis. ΔCt and ΔΔCt values were calculated as follows, and expression levels were analyzed using the 2-ΔΔCt method: ∆Ct = Ct (gene of interest) - Ct (housekeeping gene) ΔΔCt = ∆Ct (treated sample) - ∆Ct (untreated sample) Higher ΔCt values indicate lower expression levels.
次要结局
- BMI(1 day)
研究者
Elif Yıldırım Ayaz
Assoc. Prof. Dr.
Sultan Abdulhamid Han Training and Research Hospital, Istanbul, Turkey