Study of CD184 in Systemic Lupus Erythematosus
试验速览
- 阶段
- 不适用
- 入组人数
- 100
- 主要终点
- changes in the expression of CD184(CXCR4) expression in circulating B cells
研究概览
简要总结
The objective of this study is to estimate the possible role of CD184 in the pathogenesis of SLE; comparing its level among SLE cases to healthy controls.
详细描述
Detection of CXCR4 (CD184) expression on CD19+ B cells by flow cytometry in patients of SLE, comparing its level in the development of lupus activity and pathogenic process of SLE internal organ involvement/ mucocutaneus and/or musculoskeletal involvement. Identify this correlation will be useful for clarifying other accessory factors to improve the diagnosis, better treatment strategy, follow up as well as prognosis for these patients
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 16 Years 至 60 Years（Child, Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Confirmed SLE patients diagnosed and fulfilled the SLE classification criteria according to the 2019 ACR/EULAR classification criteria for SLE (Aringer et al., 2019)
- •Willing and agreed to be included in the study.
排除标准
- •Childhood SLE.
- •SLE-patients will refuse to consent to this study.
- •Other autoimmune diseases.
- •Known or suspected malignancies, especially B cell lymphoma.
结局指标
主要结局
changes in the expression of CD184(CXCR4) expression in circulating B cells
时间窗: 6 months
Flow cytometry analysis of chemokine receptor (CXCR4):Human peripheral-blood mononuclear cells (PBMCs) labeling with CXCR4 antibody will be performed in the same day after taking blood samples and subsequent measurement of the CXCR4 mean fluorescence intensity (MFI) by flow cytometry. MFI of the anti-chemokine receptor (CXCR4) staining will be calculated according to statistical thresholds set in reference to staining with negative control antibodies. The patient's CXCR4 on CD19+ B cells expression or mean MFI will be further compared to the MFI of the simultaneously performed age-matched controls samples and other subgroups of SLE patients.
次要结局
未报告次要终点
研究者
Asmaa Abdelfattah Ibrahim
the Master Degree in Clinical and Chemical Pathology
Sohag University