Establishment of Small Intestinal Human Organoids to Check the Influence of Nutrient Antigens or Therapeutic Agents
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 发起方
- 入组人数
- 375
- 试验地点
- 1
- 主要终点
- Determination of proliferation, apoptosis, histology, and cytokine Expression before and after Antigen provocation of organoids with gluten peptides, nutritional Antigens, Glucagon like peptide antagonist, or Tumor nekrose factor antagonists
研究概览
简要总结
The study evaluates the effect of nutrient antigens or therapeutic agents on human small intestinal organoids.
详细描述
Small intestinal biopsies will be taken to establish human intestinal organoids in vitro. The organoids will be propagated and various antigens will be checked for their effects on proliferation, production of inflammatory components or signal cascade.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •patients with gastrointestinal disorders and therapeutical indication of gastroduodenoscopy or coloscopy
- •healthy controls for preventive medical check-up
排除标准
- •pregnant women
结局指标
主要结局
Determination of proliferation, apoptosis, histology, and cytokine Expression before and after Antigen provocation of organoids with gluten peptides, nutritional Antigens, Glucagon like peptide antagonist, or Tumor nekrose factor antagonists
时间窗: baseline vs 24h vs 48h vs 72h
Determination of effects on organoids after antigen Stimulation with ELISA, histochemistry, RNA or proteome analysis
次要结局
未报告次要终点