Aerobic Exercise-Induced Changes in Ferritin, Folate, Vitamin B12, and Parathyroid Hormone Levels in Professional Soccer Players
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 40
- 试验地点
- 1
- 主要终点
- Change in Serum Ferritin Concentration From Pre-Exercise to Immediately Post-Exercise
研究概览
简要总结
This study aims to investigate acute changes in serum ferritin, folate, vitamin B12, and parathyroid hormone (PTH) levels following aerobic exercise in professional soccer players. Twenty professional male soccer players will voluntarily participate in the study. Participants will complete a progressive 20-meter shuttle run test. Venous blood samples will be collected immediately before and after the exercise protocol. Pre- to post-exercise changes in each blood parameter will be analyzed to determine the acute physiological responses to aerobic exercise in professional soccer players.
详细描述
This single-group interventional study is designed to examine acute changes in selected blood biomarkers following aerobic exercise in professional soccer players. A total of 20 professional male soccer players aged 18-25 years will voluntarily participate. Participants will be recruited from a professional soccer club in Karabuk, Türkiye. Before participation, all individuals will receive information about the study procedures, provide written informed consent, and undergo medical screening to determine their eligibility for maximal aerobic exercise.
Body height and body mass will be measured before exercise testing. Aerobic exercise will be performed using a progressive 20-meter shuttle run test. Participants will run continuously between two lines positioned 20 meters apart while following prerecorded audio signals. Running speed will progressively increase as the interval between the audio signals decreases. The test will end when a participant fails to reach the designated line before the audio signal on two consecutive occasions.
Venous blood samples of approximately 5 mL will be collected immediately before and immediately after the exercise protocol by qualified healthcare personnel. Blood samples will be collected into anticoagulant-free serum tubes. Following a clotting period of approximately 30 minutes, the samples will be centrifuged at 3,000 revolutions per minute to separate the serum. Serum samples will be stored at -70°C until laboratory analysis.
Serum ferritin, folate, vitamin B12, and parathyroid hormone (PTH) concentrations will be measured. Pre- to post-exercise changes in each biomarker will be analyzed separately to determine the acute physiological responses elicited by aerobic exercise in professional soccer players.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
盲法说明
No masking was applied in this single-group exercise intervention study.
入排标准
- 年龄范围
- 18 Years 至 25 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Professional soccer players.
- •Willing and able to participate in the study procedures.
- •Provision of informed consent.
排除标准
- •Inability to complete the aerobic exercise protocol.
- •- Withdrawal from the study or unwillingness to continue participation.
研究组 & 干预措施
Aerobic Exercise
All participants will complete a progressive 20-meter shuttle run test. Venous blood samples will be collected immediately before and immediately after the exercise protocol to assess acute changes in serum ferritin, folate, vitamin B12, and parathyroid hormone concentrations.
干预措施: 20-Meter Shuttle Run Test (Behavioral)
结局指标
主要结局
Change in Serum Ferritin Concentration From Pre-Exercise to Immediately Post-Exercise
时间窗: Immediately before exercise and immediately after completion of the 20-meter shuttle run test
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum ferritin concentration will be measured in ng/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Change in Serum Folate Concentration From Pre-Exercise to Immediately Post-Exercise
时间窗: Immediately before exercise and immediately after completion of the 20-meter shuttle run test
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum folate concentration will be measured in ng/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Change in Serum Vitamin B12 Concentration From Pre-Exercise to Immediately Post-Exercise
时间窗: Immediately before exercise and immediately after completion of the 20-meter shuttle run test
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum vitamin B12 concentration will be measured in pg/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Change in Serum Parathyroid Hormone Concentration From Pre-Exercise to Immediately Post-Exercise
时间窗: Immediately before exercise and immediately after completion of the 20-meter shuttle run test
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum parathyroid hormone (PTH) concentration will be measured in pg/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
次要结局
未报告次要终点
研究者
Assoc. Prof. Dr. Özgür EKEN
Assoc. Prof.Dr.
Inonu University