Role of the BACE1-AS Long Noncoding RNA in Ischemic Heart Failure
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 630
- 试验地点
- 1
- 主要终点
- Peripheral blood RNAs expression in ACS
研究概览
简要总结
The objective of this project is 1) to explore the clinical relevance of BACE1-AS and BACE1 as therapeutic targets and 2) to evaluate their potentiality as biomarkers in ischemic heart failure (HF).
The first aim will be studied by validating in left ventricle biopsies in patients with post-ischemic heart failure the transcriptome and DNA methylation status data obtained in cell lines where the expression of BACE1-AS has been modulated.
The investigation of BACE1-AS and BACE1 as biomarkers will be obtained by using peripheral blood mononuclear cells (PBMCs) from patients with acute coronary syndrome (ACS) treated with percutaneous angioplasty (PCI) procedure and undergoing to left ventricular remodeling. Furthermore, PBMCs from patients with chronic ischemic HF recruited from the Istituti Clinici Scientifici Maugeri Pavia will be also used. From the data obtained from these patients and from non-decompensated subjects, the levels of BACE1-AS and BACE1 in the blood will be measured and correlated with each other and with β-amyloid levels (βA), as well as with clinically relevant parameters.
详细描述
1.1) Validation of transcriptomic data obtained in vitro: The transcriptome of the AC16 cardiomyocyte cell line in which the expression of BACE1-AS was induced by lentivirus vectors will be analyzed, thus identifying the transcripts specifically modulated by BACE1-AS. The data obtained in vitro will be validated by analyzing the RNAs extracted from 20 HF patients and 20 controls. These RNA samples are already available thanks to the RNA_HF_AS project "Study of the transcriptome in heart failure and aortic stenosis" (San Raffaele Hospital - Milan, Ethics Committee CE 85/int/2016).
1.2) Validation of epigenetic data: The effect of BACE1-AS on the state of DNA methylation will be studied in the AC16 cardiomyocyte cell line. In over-expressing BACE1-AS cells, DNA hypomethylation will be evaluated by MS-HRM and pyrosequencing studies of bisulfite-modified genomic DNA of 69 enhancer sequences identified by us as candidates in preliminary studies. The data thus obtained will be validated in 20 cardiac biopsies from patients with post-ischemic HF and in 20 controls. Left ventricular biopsies will be taken after obtaining informed consent from 20 patients suffering from non-terminal dilated cardiomyopathy (DCM) undergoing left ventricular reconstruction (SVR) surgery at the San Donato Policlinico. A sufficient number of samples with these characteristics have already been obtained from IRCCS Policlinico San Donato as part of the RNA_HF_AS project (San Raffaele Hospital - Milan, Ethics Committee CE 85/int/2016). and not completely used for the study.
1.3) Study of the expression levels of BACE1-AS, BACE1 in PBMC of patients with ischemic cardiomyopathy.
The expression levels of BACE1 and BACE1-AS will be measured in PBMCs from the following groups:
Group 1:
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 75 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •left ventricular reconstruction surgery (SVR)
- •ESVI >35 ml/m2
- •previous anterior transmural infarction
- •age 40-75 years
排除标准
- •ESVI <35 ml/m2
- •Tempo from E unknown
- •Pregnant women
- •Patients with genetic diseases
- •Patients suffering from malignant neoplasm
- •Subjects with collagenopathies
- •Subjects undergoing chemo/radiotherapy
- •Subjects undergoing prolonged steroid therapy
- •Subjects with known active infectious diseases
- •Subjects with positive serology for HIV, HBV (not vaccinated), HCV.
- •Patients with ACS and left ventricular dysfunction 12 months after percutaneous angioplasty
- •Inclusion criteria:
- •Age >18 years
- •Patients with ACS (first episode), defined according to the ESC 2017 guidelines [19]
- •Informed consent to enrollment in the study
- •Exclusion Criteria:
- •Severe heart valve disease or other conditions requiring cardiac surgery
- •Previous cardiac surgery including coronary artery bypass grafting
- •Total chronic occlusions
- •Patients with known hypersensitivity or contraindication to any of the following drugs:
- •heparin aspirin, clopidogrel, ticlopidine, sirolimus, everolimus.
- •Any contraindication to implantation of drug-eluting stents (DES)
- •Patients with previous documented myocardial infarction;
- •LVEF <30% before PCI
- •Patients in cardiogenic shock
- •Patients with advanced STEMI (> 48 h from the onset of symptoms/Q waves on ECG) or undergoing fibrinolysis;
- •Patients with known previous cardiomyopathy with LVEF < 40%
- •Patients suffering from malignant neoplasms or systemic pathologies with a quoad vitam prognosis of less than 1 year;
- •Patients suffering from known active infectious diseases;
- •Patients who are unable to express valid informed consent at the time of enrollment
- •Pregnant women
- •Patients with chronic post-ischemic HF ( recruitment at the Istituti Clinici Scientifici Maugeri Pavia)
- •Inclusion criteria:
- •All patients admitted to Rehabilitation Cardiology at the Cardiology Department of ICS Maugeri with a diagnosis of chronic heart failure on an ischemic or "acute" basis due to exacerbation of chronic heart failure due to previous ischemia and whose therapies have been optimized at the moment of enlistment.
- •NYHA Class II-III at the time of enlistment;
- •Age over 18 years;
- •Informed consent signed at the time of enrollment in the study.
- •Exclusion criteria:
- •Denial of informed consent;
- •End-stage renal disease;
- •Presence of ACS, stroke, transient ischemic attack, major cardiovascular surgery, percutaneous coronary angioplasty or carotid angioplasty in the previous 3 months;
- •Patients undergoing cardiac transplantation or on the active list for cardiac transplantation or undergoing LVAD implantation;
- •Presence of hemodynamically significant mitral or aortic valve disease except mitral insufficiency due to ventricular dilatation;
结局指标
主要结局
Peripheral blood RNAs expression in ACS
时间窗: From year 1 to year 3
The RNAs expression in peripheral blood mononuclear cells of acute coronary syndrome patients treated with percutaneous angioplasty and remodelling compared to controls
Cardiac tissutal RNAs expression
时间窗: From year 1 to year 3
The RNAs cardiac tissutal expression of non- and end-stage heart failure patients compared to controls
Peripheral blood RNAs expression in heart failure
时间窗: From year 1 to year 3
The RNAs expression in peripheral blood mononuclear cells of heart failure patients compared to controls
次要结局
未报告次要终点
研究者
Fabio Martelli
Director of Molecular Cardiology Laboratory
IRCCS Policlinico S. Donato