The Role of Glycocalyx and Oxidative Stress in Endothelial Function (Part 2)
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 35
- 试验地点
- 1
- 主要终点
- Endothelial glycocalyx
研究概览
简要总结
The primary research objective of the project is to determine the role of eGC in microvascular reactivity in patients with coronary heart disease (CHD) before and after bypass grafting (cross-sectional study 2).The main objective of the project is to increase the excellence and interdisciplinarity of scientific work at MEFOS by connecting researchers from different scientific fields, improving conditions and resources for scientific work, and increasing international cooperation, in line with strategic objective 1: raising scientific excellence. The results of the project could provide pathophysiological insight into the development and maintenance of endothelial dysfunction in CVD, as well as identify new biomarkers for CVD.
详细描述
The primary objective of this project is to determine the association between endothelial glycocalyx (eGC) and myocardial revascularization in patients undergoing coronary artery bypass grafting and clinical outcomes of treatment (observational prospective study 2).
Specific objective 2.1: Measure eGC thickness in coronary artery disease patients before and after surgery.
Specific objective 2.2: Determine the reactivity of the bypass graft sample in in vitro experiments.
Specific objective 2.3: Quantify soluble markers of eGC, oxidative stress, and inflammation from serum and PBMC samples, as well as antioxidant capacity.
Specific objective 2.4: Determine the association between the results of functional tests, eGC, and markers from specific objective 2.3 with clinical outcomes of treatment.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •adult patients of both sexes over 18 years of age
- •Caucasian
- •undergoing elective coronary artery bypass grafting (CABG)
- •provided written informed consent
排除标准
- •emergency surgery
- •sepsis or severe systemic inflammation before surgery
- •severe renal or hepatic insufficiency
- •immunosuppression
- •serious comorbidities (e.g., active cancer)
- •therapies that could affect oxidative stress (e.g., high doses of corticosteroids)
研究组 & 干预措施
Coronary Heart Disease (CHD) patients
Adult patients of both sexes over 18 years of age, undergoing elective coronary artery bypass grafting (CABG) and who provided written informed consent
结局指标
主要结局
Endothelial glycocalyx
时间窗: Perioperative/Periprocedural
Measurement of the thickness of endothelial glycocalyx in coronary heart disease patients before and after bypass surgery - measured by Glycocheck
次要结局
- Bypass graft sample reactivity(Baseline, at one time point)
- Endothelial glycocalyx soluble markers(Perioperative/Periprocedural)
- Inflammation(Perioperative/Periprocedural)
- Antioxidant capacity(Perioperative/Periprocedural)
- Intracellular oxidative stress production(Perioperative/Periprocedural)
- Oxidative stress biomarkers(Perioperative/Periprocedural)
研究者
Ines Drenjancevic
Head of Department of Physiology and Immunology Faculty of Medicine Osijek
Josip Juraj Strossmayer University of Osijek