Effects of High Intensity Interval Training and Moderate Intensity Continuous Training on Nitric Oxide-mediated Erythrocyte Rheology
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 60
- 试验地点
- 2
- 主要终点
- Intracellular NO production response to exercise training.
研究概览
简要总结
Erythrocyte rheological properties affect blood viscoelasticity and consequently regulate vascular resistance to flow shear force, whereas rheological impairments of erythrocytes may result in circulatory disorders. The aim of this study was to establish an effective exercise strategy for improving individual aerobic capacity and for simultaneously ameliorating the risk of hemorheological dysfunction evoked by a graded exercise test (GXT) and the hypotheses is exercise intervention will improved hemorheological functions by enhancing deformability of erythrocytes via NO-mediated mechanism. This study included 60 healthy sedentary mens (age 20~30) from Chang Gung university than were randomized into the HIIT [3-min intervals at 40% and 80% V̇O2 reserve (V̇O2R),n=10] and MICT(sustained 60% V̇O2R,n=10)on a bicycle ergometer for 30min·d-1, 5 d·wk-1 for 6 wk.
详细描述
Recently, the role of erythrocyte has been more emphasized, which also related with endothelial cell. For coronary artery patients, the endothelial nitric oxide synthase activity in red blood cell (RBC-eNOS activity) is lower than age-matched health people, and it is related with dysfunction of endothelial cell. In cardiovascular diseases. the erythrocyte arginase-1 is active and seize L-arginine with eNOS. When the Arg-1 stimulated by reactive oxygen species (ROS), the nitric oxide (NO) bioactivity decrease and produce more ROS, meanwhile, ROS can go around to stimulate Arg-1. When the RBC-NO production is lowering, it will increase the adhesion activity to endothelial cell due to erythrocyte can be quite close to blood vessel well then release Nitric Oxide, induce the dysfunction and oxidative pressure of endothelial cell. The NO can also regulate the deformability of erythrocytes, and extremely affect oxygen supply to tissue once the deformability and aggregation of erythrocyte become abnormal. Besides NO, the deformability will be affected if erythrocyte is continuously exposed to the endogenous or exogenous ROS, which also increase adhesion to endothelial cell with the exposure of phosphatidylserine. Exercise can regulate the mechanism of NO release from erythrocyte, affecting the rheology of erythrocyte, and improve the anti-oxidation ability. Therefore, as mentioned above which make erythrocyte, as many aspects, become an important role on atherosclerosis disease treatment.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Outcomes Assessor)
入排标准
- 年龄范围
- 20 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Having a sedentary lifestyle (without regular exercise, exercise frequency ≤ once weekly, duration < 20 min).
排除标准
- •Exposed to high altitudes (> 3000 m) for at least 1 year.
- •Taking medications or vitamins
- •Having any cardiopulmonary/hematological risk.
结局指标
主要结局
Intracellular NO production response to exercise training.
时间窗: 8 weeks
Added different inhibitors or agonists to investigate the effects of exercise training on NO production mediated by eNOS-NO pathway.
Intracellular ROS production response to exercise training.
时间窗: 8 weeks
Added different inhibitors or agonists to investigate the effects of exercise training on ROS production mediated by eNOS-NO pathway.
The levels of eNOS, p-eNOS and Band-3 response to exercise training.
时间窗: 8 weeks
Detect the following protein levels: eNOS, p-eNOS and Band-3, by the western blots.
次要结局
- Erythrocyte deformability(8 weeks)
- Cardiopulmonary fitness(8 weeks)
- Determination of erythrocyte biological markers by Flow Cytometry(8 weeks)
研究者
Jong-Shyan Wang
Principal Investigator
Chang Gung Memorial Hospital