DNA Damage and Oxidative Stress in Hospitalized COVID-19 Patients Compared to Healthy Controls: A Case-control Study
试验速览
- 阶段
- 不适用
- 入组人数
- 135
- 试验地点
- 1
- 主要终点
- DNA damage
研究概览
简要总结
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the coronavirus disease 2019 (COVID-19) pandemic challenging health systems worldwide.
While there is a clear correlation between oxidative stress markers and the severity of many viral diseases such as hepatitis C, for SARS-CoV clinical data is limited.
The investigators aim at 1.) investigating DNA damage, oxidative stress, inflammation, and aging markers in COVID-19 patients and compare them with age and gender matched healthy controls and patients with influenza; and 2.) investigating all aforementioned parameters during "cytokine storm" via repeated blood sampling.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 40 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •gender: female/male
- •age: ≥40 years
- •patients admitted at the emergency department
- •COVID-19 suspected patients
- •able to give written informed consent
排除标准
- •children (≤ 18 years) and adults 19-39 years
- •not hospitalized, but confirmed COVID-19 patients (outpatients)
- •patients without definitive COVID-19 confirmation
- •Control group:
- •Inclusion criteria:
- •gender: female/male
- •age: ≥40 years
- •absence of severe illnesses/serious diseases
- •able to give written informed consent
- •Exclusion criteria:
- •children (≤ 18 years) and adults 19-39years
- •any current or past clinically significant pathology/disease (comorbidity) such as:
- •Malignancies
- •Cardiovascular diseases
- •Diabetes mellitus (I and II)
- •Obesity class III (BMI >40)
- •Other severe diseases (e.g. renal, hepatic, thyroid disease, bone metabolic diseases, rheumatoid osteoarthritis, human immunodeficiency syndrome, ...)
- •Influenza group:
- •Inclusion criteria:
- •gender: female/male
- •age: ≥40 years
- •patients admitted at the emergency department
- •influenza suspected patients
- •able to give written informed consent
- •Exclusion criteria:
- •children (≤ 18 years) and adults 19-39 years
- •patients without definitive influenza confirmation
结局指标
主要结局
DNA damage
时间窗: Baseline
Compare DNA damage (% of DNA in the tail; measured by the comet assay) between hospitalized COVID-19 patients to influenza patients and healthy controls.
次要结局
- The investigators will consider RDA and DNA gene expression(Baseline)
- The investigators will consider oxidative stress marker(Baseline)
- The investigators will consider inflammatory marker(Baseline)
- The investigators will consider clinical biochemistry marker(Baseline)
- The investigators will consider further DNA damage parameter(Baseline)
- The investigators will consider the phenotypic age marker(Baseline)
研究者
Wagner Karl-Heinz
Professor of Human Nutrition
University of Vienna