Preliminary Study of Sonic Hedgehog Signaling Pathway in the Pathogenesis of Rheumatoid Arthritis
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 78
- 主要终点
- mRNA expression
研究概览
简要总结
Rheumatoid arthritis(RA) with a high incidence and high morbidity, the pathogenesis has not been fully elucidated. Fibroblast-like synovial cells excessive proliferation and synovial angiogenesis is the most important cause of RA synovitis and joint destruction. Our study was to find the role of Sonic Hedgehog(SHH) pathway in regulating proliferation of fibroblast-like synovial cells and modulating excess angiogenesis of synovial tissue.
详细描述
4ml blood from active RA patients(n=35) and healthy volunteers (n=35) will be collected
- peripheral blood mononuclear cells will be detached
- messenger ribonucleic acid expression of Sonic Hedgehog pathway associated factors in both groups will be detected
Synovial tissues from 4 RA patients and 4 patients with traumatic or meniscal injury who need to carry out knee joint replacement operation will be collected
- inflammatory feature of synovial tissue will be observed
- the expression of Shh, Ptch1, Gli1 and Smo in synovial tissue will be detected by immunohistochemistry assay
- fibroblast-like synoviocytes will be cultured from synovial tissue of RA patients
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •definitely diagnosed
- •the disease is active (DAS28>3.2)
排除标准
- •combined with severe organ dysfunction
- •combined with other rheumatic diseases
结局指标
主要结局
mRNA expression
时间窗: 1 day (December 15, 2012)
次要结局
未报告次要终点
研究者
Shangling Zhu
Post-Graduate
Third Affiliated Hospital, Sun Yat-Sen University