The Effect of a Retrofit Particle Trap on the Vascular Effects of Diesel Exhaust Inhalation
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 18
- 试验地点
- 2
- 主要终点
- Forearm blood flow in response to infused intra-arterial vasodilators
研究概览
简要总结
The purpose of this study is to determine whether a retrofit particle trap can reduce the adverse vascular responses to diesel exhaust inhalation
详细描述
18 subjects healthy male volunteers will be recruited at Umeå University. In a randomised, double blind 3 way crossover trial, subjects will be exposed to filtered air, diesel exhaust (300mcg/m3) or filtered diesel exhaust for 1 hour during intermittent exercise.
2 hours following the exposure, thrombogenicity will be assessed using the Badimon chamber - an ex-vivo model of thrombosis formed under constant flow conditions.
Forearm blood flow in response to infused intra-brachial vasodilators will be measured using venous occlusion plethysmography 6 hours after the exposure.
Arterial stiffness will be measured using peripheral arterial applanation tonometry in the hour post-exposure.
Blood samples will be collected at timepoints over the 24 hours after exposure.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Healthy male volunteers
排除标准
- •Intercurrent illness
- •Significant occupational exposure to air pollution
- •Regular medication usage
结局指标
主要结局
Forearm blood flow in response to infused intra-arterial vasodilators
时间窗: 6 hours post-exposure
次要结局
- Arterial stiffness measured by applanation tonometry(In the 1 hour following exposure)
- Thrombogenicity measured using the Badimon chamber - an ex-vivo model of thrombosis under conditions of continuous flow(2 hours after the exposure)
- Exhaled nitric oxide - a marker of pulmonary inflammation(1 hour & 6 hours after exposure)
- Endogenous fibrinolytic capacity - measured as net release of t-PA in response to infused bradykinin(6 hours after exposure)
- Biochemical markers of systemic inflammation(Baseline, 2, 6 & 24 hours)