Role of Cysteinyl Leukotrienes in the Pathogenesis of Asthma in Obesity
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 117
- 试验地点
- 1
- 主要终点
- Urine Cysteinyl Leukotriene Per Creatinine
研究概览
简要总结
The purpose of this study is to examine whether there are higher levels of cysteinyl leukotrienes in obese subjects than in non-obese subjects. Cysteinyl leukotrienes are pro-inflammatory substances that cause asthma by narrowing the airways of the lung. The investigators want to see if subjects with increased fat stores and therefore increased leptin, which is a fat-related protein that regulates leukotrienes, have increased levels of leukotrienes in the blood, lung and urine. The investigators would also like to determine the relationship between cysteinyl leukotrienes and exhaled nitric oxide levels in asthmatics with and without obesity. Nitric oxide is anti-inflammatory and suppresses leukotriene synthesis.
详细描述
This is a cross-sectional observational study to characterize the association between obese asthmatics and the inflammatory mediators cysteinyl leukotrienes. Our hypothesis is that altered adipokine levels in obesity result in augmented cysteinyl leukotriene synthesis that play a significant role in the pathogenesis of asthma in these subjects.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •18-65 yr old adults with asthma, non-smoking for at least one year
- •Healthy non-smoking adults, aged 18-65 without asthma
- •Heavy subjects with BMI > 30 kg/m2
- •Subjects with normal weight (BMI between 20-25 kg/m2)
- •Subject not taking anti-leukotriene therapy (Singulair, Accolate, Zyflo)
排除标准
- •No chronic oral steroid use
- •No recent history of infection
- •No recent history of flare of lung disease
结局指标
主要结局
Urine Cysteinyl Leukotriene Per Creatinine
时间窗: sample taken over 5 minutes or less
Urine inflammatory mediators measured from a single urine sample
Ratio U Cys-LT/FeNO
时间窗: sample taken over 5 minutes or less
Responsiveness to leukotriene modifier medication by measuring the urine cysteinyl leukotriene/exhaled nitric oxide ratio
Exhaled Nitric Oxide (FeNO)
时间窗: Observational: Two exhalations within 1 minute
Exhaled breath nitic oxide ppb (averaged values from 2 exhalations per participant)
Plasma Leptin
时间窗: Observational: one time point from a blood draw after more than 6 hours fasting
Measurement of plasma leptin in obese and non obese asthmatics and non-asthmatic subjects from a single blood draw.
次要结局
未报告次要终点
研究者
Michael Coffey
Associate Professor Internal Medicine, Pulmonary Division
University of Michigan