Assessment of Correlation of Red Complex Bacteria in Saliva and Gingival Crevicular Fluid(GCF) using Multiplex Real Time – Polymerase Chain Reaction(RT-PCR) In Subjects with Periodontitis.

Periodontitis is a chronic inflammation associated with dysbiotic subgingival biofilms , resulting in progressive and irreversible destruction of the tooth supporting tissues.Although it is not clear whether dysbiotic biofilms initiate the disease or is a consequence of the disease, it is well established that at diseased status the subgingival microbiota is enriched with gram-negative, proteolytic bacteria, while in healthy situation, the microbiota is mainly composed of gram-positive bacteria.Among microbial complexes, the first complex that has been related to disease is the orange complex consisting of anaerobic gram-negative species (Prevotella intermedia, Prevotella nigrescens, Prevotella micros, and Fusobacterium nucleatum) which on disease progression shifts toward red-complex bacteria consisting of Tannerella forsythia, Treponema denticola, and Porphyromonas gingivalis.The red complex bacteria play a critical role in the pathogenesis of periodontitis. These bacteria are found together in periodontal pocket, implicating that they work in synergistic manner leading to destruction of periodontal tissue. The prevalence of periodontitis in India is 51%, and studies related to diagnosis of different forms of Periopathogens are few in Indian published literature, hence its also necessary to further establish data regarding the same.Hence an attempt is being made to assess and correlate in both saliva and GCF the red complex bacteria Porphyromonas Gingivalis (Pg), Tannerella Forsythia (Tf) & Treponema Denticola (Td) using Multiplex RT-PCR using saliva and GCF samples in periodontitis patients.