Probing the Salivary Proteome for Prognostic Biomarkers in Response to Non-Surgical Management of Periodontitis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- Saliva protein content change
研究概览
简要总结
This study aims to investigate salivary proteome changes in periodontitis patients before and after non-surgical treatment. Ten systemically healthy and non-smoker individuals with stage III, grade C periodontitis underwent non-surgical periodontal treatment. Saliva was collected at baseline, and one and six months post-treatment. Whole-mouth plaque and gingival index, probing depth, bleeding on probing and clinical attachment loss were measured. The saliva proteome was investigated by label-free quantitative proteomics. Normalized protein intensities were measured and protein changes were modeled over time with significant protein regulation considered at false discovery rate (FDR)<0.05.
详细描述
Study Population and Clinical Examination
A total of 10 systemically healthy and non-smoker individuals with stage III, grade C periodontitis were recruited at the Department of Periodontology, School of Dentistry, Aydın Adnan Menderes University, Aydın, Turkey.
Full-mouth periodontal assessment included measurements of probing depth, clinical attachment loss, the percentage of sites with bleeding on probing, gingival index, and plaque index at baseline (T0) and one month (T1) and six months (T6) post-treatment. All measurements were obtained from 6 sites per tooth excluding third molars using a manual periodontal probe (William's periodontal probe, Hu-Friedy, Chicago, IL). The alveolar bone resorption was assessed on the digital panoramic radiograph in each participant. All clinical measurements were performed by an experienced periodontist (B.A.).
Diagnosis of stage III, grade C periodontitis was performed according to the criteria proposed by the 2017 International Workshop on the Classification of Periodontal and Peri-implant Diseases and Conditions.
Treatment
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Prospective
入排标准
- 年龄范围
- 32 Years 至 43 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Non-smokers
- •Having at least 20 natural teeth.
排除标准
- •Having any chronic inflammatory or immunological conditions such as diabetes mellitus, cardiovascular diseases, rheumatoid arthritis and mucocutaneous diseases,
- •Pregnant or lactating
- •Intake of antimicrobials, anti-inflammatory drugs and immunosuppressive agents within the last 6 months
- •Non-surgical/surgical periodontal therapy within 12 months
- •Having orthodontic appliances, removable partial dentures and restorative and endodontic treatment requirements.
结局指标
主要结局
Saliva protein content change
时间窗: Change from baseline to 1 month and 6 months after treatment
pg/ml
次要结局
未报告次要终点
研究者
Beral Afacan
Principal Investigator
Aydin Adnan Menderes University