Tissue Resident Memory T Cells in Early & Late Cases of Generalized Non-segmental Vitiligo
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 29
- 试验地点
- 1
- 主要终点
- Double CD8+ and CD69+ immunofluorescence
研究概览
简要总结
This study aims to detect the presence and diversity of Tissue resident memory T cell populations in early and late cases of generalized non-segmental Vitiligo.
详细描述
- Written informed consent obtained from every participant.
- Detailed history taking including onset, course, duration of the disease, aggravating factors and received medications
- VASI score assessed in every patient.
- Patients photographed, divided into two groups early & late cases and 3 mm punch biopsies will be taken from perilesional and non lesional skin.
- Biopsies processed and stained for CD8 & CD69 in cases of early group, & late group and controls by immunofluorescence.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •- Patients with Generalized non-segmental Vitiligo ( lesions in more than one anatomical site ).
- •Both genders.
- •Early group: onset less than or equal 6 months.
- •Late group: onset more than 6 months
排除标准
- •Localized or Segmental Vitiligo.
- •Age < 14 years.
- •Pregnant females
- •Patient who received treatment of any type within the last 3 months for the late group.
- •Patients with other skin diseases or immunosuppression
结局指标
主要结局
Double CD8+ and CD69+ immunofluorescence
时间窗: 6months-1 year
Number of Tissue resident Memory cells by double stained by CD8+ \&CD 69+ in early and late generalized non segmental vitiligo patients
CD69+ immunofluorescence
时间窗: 6months-1 year
Number of Tissue resident Memory cells by positively stained by CD8+ in early and late generalized non segmental vitiligo patients
CD8+ immunofluorescence
时间窗: 6months-1 year
Number of Tissue resident Memory cells by positively stained by CD8+ in early and late generalized non segmental vitiligo patients
VASI
时间窗: 6months-1 year
Vitiligo Area and Severity index minimum 0 maximum 100, the larger the number the worse the condition
次要结局
未报告次要终点
研究者
Rana F Hilal, MD
Assistant Professor
Kasr El Aini Hospital