Staphylokinase and ABO Group Phenotype: New Players in Staphylococcus Aureus Implant-associated Infections Development
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 88
- 试验地点
- 1
- 主要终点
- IAI and NC nasal carriage isolates genetic background
研究概览
简要总结
The purpose of this study is to identify bacterial and/or clinical features involved in the pathogenesis of Staphylococcus aureus implant-associated infections (IAI). Materials & methods: In total, 57 IAI S. aureus and 31 nasal carriage (NC) S. aureus isolates were studied. Staphylococcus aureus genetic background was obtained by microarray analysis. Multilocus sequence typing was performed to determine clonal complexes (CC). Biofilm production was investigated by resazurin and crystal violet methods
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Retrospective
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •infected patients with S. Aureus IAI infection
- •noninfected patients screened for S. aureus nasal colonization at the orthopedic consultation ward
排除标准
- 未提供
研究组 & 干预措施
control group
patients with Staphylococcus aureus nasal carriage
干预措施: Staphylococcus aureus nasal carriage infection (Other)
IAI patients group
patients with Staphylococcus aureus of implant-associated infections
干预措施: IAI infection (Other)
结局指标
主要结局
IAI and NC nasal carriage isolates genetic background
时间窗: september 2012
IAI and NC isolates genetic background was determined in order to compare the prevalence of individual virulence factor genes. All genes provided by the Alere StaphyType DNA microarray were studied
biofilm production measurement in the two groups
时间窗: september 2012
The primary aim of this study is to identify phenotypic (biofilm production) markers in two different groups of S. aureus strains isolated from patients with IAI or screened from nasal carrier in noninfected patients undergoing orthopedic surgery. By using resazurin method, IAI and NC nasal carriage isolates were divided into three groups. Isolates were classified as strong, moderate and weak biofilm producers.
Clonal complexes distribution
时间窗: september 2012
The primary aim of this study is to identify genotypic markers in two different groups of S. aureus strains isolated from patients with IAI or screened from nasal carrier in noninfected patients undergoing orthopedic surgery. IAI and NC isolates genetic background was determined in order to compare the prevalence of individual virulence factor genes. All genes provided by the Alere StaphyType DNA microarray were studied.
次要结局
- biofilm production correlation with genotype(december 2013)
- Association of ABO group phenotype and IAI S. aureus genotype(december 2013)
- Correlation between Clonal Complexes distribution , genotype and clinical parameters(december 2013)