Cryopreservation of Oocytes: A Randomized Controlled Trial Comparing Slow-freezing to Vitrification
试验速览
- 阶段
- 不适用
- 状态
- 终止
- 发起方
- 入组人数
- 14
- 试验地点
- 2
- 主要终点
- post-thaw oocyte survival rate
研究概览
简要总结
Preservation of fertility is limited. Current methods include embryo cryopreservation and while still experimental, ovarian cryopreservation. In single women who are at risk for infertility secondary to cancer or couples who have ethical reasons to avoid embryo cryopreservation, oocyte cryopreservation is an alternative Purpose of study is to compare slow freeze to vitrification of human oocytes. Successful cryopreservation and subsequent thawing programs are characterized by avoiding ice crystal formation.Vitrification, newer alternative to slow freeze method, process by which water is prevented from forming ice.
Our hypothesis is that higher survival rate per oocyte is to be expected in the vitrification group.
详细描述
Although several reviews of smaller studies of cryoprotectant methodology exist randomized controlled trials,comparing slow-freeze-rapid-thaw procedure to vitrification of oocytes, are not available. The purpose of this study is to compare these two methods of oocyte cryopreservation in human oocytes.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 37 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •14 or more oocytes at ovum pick up
- •age < 38
- •bmi < 30
排除标准
- 未提供
研究组 & 干预措施
1
slow-freeze
干预措施: slow-freeze (Procedure)
2
vitrification
干预措施: vitrification (Procedure)
结局指标
主要结局
post-thaw oocyte survival rate
时间窗: 2 years
次要结局
- embryo development(2 years)
- implantation rate(2 years)
- fertilization rate(2 years)
- cleavage(2 years)
- ongoing pregnancy(2 years)
研究者
Ellen Greenblatt
Associate Professor, U of T
Samuel Lunenfeld Research Institute, Mount Sinai Hospital