Collectie Van Microbioomstalen Van Huid, Mond, Vagina en Feces Van Gezonde Westerse Donoren Voor Fundamenteel en Functioneel Microbioom-onderzoek.
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 100
- 试验地点
- 2
- 主要终点
- Composition of the microbiome, as determined by metagenomics analysis of the skin, oral, vaginal and fecal microbiome samples
研究概览
简要总结
Large numbers of micro-organisms (especially bacteria) live in and on human bodies and have a very important function for the health. These microorganisms are called 'the microbiota'. They aid in the digestion of food, ensure the production of certain vitamins, and are very important for the development and regulation of the immune system. In many diseases (including Crohn's disease, arthritis, obesity, diabetes and cancer), a disruption of microbial composition is observed. There are indications that a disruption of the microbiome can contribute to the development of inflammatory diseases and cancer, but the underlying processes are not sufficiently understood. To understand the mechanisms underlying these disease processes, fundamental research is conducted at Ghent University. Stool, skin, oral and vaginal samples from various origins are examined, e.g. from people from indigenous tribes with a traditional lifestyle. It is important that these samples can be compared with microbiome samples from healthy Western (West-European) controls. In this study, the investigators want to build up a collection of samples from healthy donors between the ages of 2 and 70, with the exception of vaginal samples collected from women between the ages of 18 and 45. The samples will form the basis for further fundamental and functional research into microbiota-host interactions at Ghent University.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 2 Years 至 70 Years（Child, Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age between 2 and 70 years old
- •Dutch speaking
- •West-European origin
排除标准
- •Diagnosis of chronic disease (such as osteoarthritis, rheumatoid arthritis, diabetes, Crohn's disease, inflammatory bowel disease (IBD), asthma, COPD, etc.)
- •Antibiotics taken within 3 months before sampling
结局指标
主要结局
Composition of the microbiome, as determined by metagenomics analysis of the skin, oral, vaginal and fecal microbiome samples
时间窗: 4 years
Characterization of skin, oral, vaginal and fecal microbiome profiles from Western donors versus indigenous Brazilian tribes (Yanomami, samples collected previously)
Measurement of disease progression upon administration of different microbiota (Western versus indigenous) by weight measurement
时间窗: 4 years
Measurement of disease progression in arthritis, colorectal cancer, intestinal inflammation, obesity, etc. by measuring weight loss or gain.
Measurement of disease progression upon administration of different microbiota (Western versus indigenous) by detecting blood in stool
时间窗: 4 years
Measurement of disease progression in colorectal cancer, intestinal inflammation, etc. by determining presence of blood in stool samples.
Measurement of disease progression upon administration of different microbiota (Western versus indigenous) by serum glucose measurement
时间窗: 4 years
Measurement of disease progression in obesity by measuring serum glucose concentrations.
Measurement of disease progression upon administration of different microbiota (Western versus indigenous) by determining stool consistency
时间窗: 4 years
Measurement of disease progression in colorectal cancer, intestinal inflammation, etc. by determining stool consistency.
Compostition of the metabolome, as deteremined by metabolomics analysis skin, oral, vaginal and fecal microbiome samples
时间窗: 4 years
Characterization of skin, oral, vaginal and fecal metabolome profiles from Western donors versus indigenous Brazilian tribes (Yanomami, samples collected previously)
Measurement of immune activation upon administration of different microbiota (Western versus indigenous).
时间窗: 4 years
Measurement of immune cell composition by means of flow cytometry analysis.
Identification of the micro-organisms from different microbiota (Western versus indigenous) linked to disease progression.
时间窗: 4 years
Identification of microorganisms involved in disease models (including arthritis, colorectal cancer, intestinal inflammation, obesity, etc.) by means of in-vitro and in-vivo models. The stool samples are administered to mouse models of various diseases via oral gavage, after which the disease process is closely studied. For the in-vitro models, these samples are used to stimulate cell lines to investigate the effect on, for example, T cell activation, macrophage polarization or general cell proliferation.
次要结局
未报告次要终点